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Spirodela (duckweed) as an alternative production system for pharmaceuticals: a case study, aprotinin
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Spirodela (duckweed) as an alternative production system for pharmaceuticals: a case study, aprotinin
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Journal Article
Spirodela (duckweed) as an alternative production system for pharmaceuticals: a case study, aprotinin
2008
Overview
Aprotinin is a small serine protease inhibitor used in human health.
Spirodela
were transformed, via
Agrobacterium
, with a synthetic gene encoding the mature aprotinin sequence and a signal peptide for secretion which was driven by the CaMV 35S promoter. A total of 25 transgenic
Spirodela
lines were generated and aprotinin production was confirmed by northern and western blot analyses. Expression levels of up to 3.7% of water soluble proteins were detected in the plant and 0.65 mg/l in the growth medium. In addition, immunoaffinity purification of the protein followed by amino acid sequencing confirmed the correct splicing of the aprotinin produced in
Spirodela
and secreted into the growth medium.
Publisher
Springer Netherlands,Springer,Springer Nature B.V
Subject
/ Animal Genetics and Genomics
/ Araceae
/ Araceae - growth & development
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ drugs
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Plant
/ genetically modified organisms
/ genetics
/ Methods. Procedures. Technologies
/ Plants, Genetically Modified
/ Plants, Genetically Modified - genetics
/ Plants, Genetically Modified - growth & development
/ Plants, Genetically Modified - metabolism
/ Plasmids
/ Protein Sorting Signals - genetics
/ proteins
/ Recombinant Proteins - genetics
/ Recombinant Proteins - isolation & purification
/ Recombinant Proteins - metabolism
/ Transgenic animals and transgenic plants
