Journal Article

Irreversible electroporation promotes a pro-inflammatory tumor microenvironment and anti-tumor immunity in a mouse pancreatic cancer model

Imran, Khan Mohammad, Hendricks-Wenger, Alissa, Aycock, Kenneth N., Tintera, Benjamin, Davalos, Rafael V., Coutermarsh-Ott, Sheryl, Markov Madanick, Justin, Orr, Katie, Salameh, Zaid S., Eversole, Paige, Brock, Rebecca M., Allen, Irving C., Alinezhadbalalami, Nastaran, Powar, Manali, Beitel-White, Natalie

2024

Overview

Pancreatic cancer is a significant cause of cancer-related mortality and often presents with limited treatment options. Pancreatic tumors are also notorious for their immunosuppressive microenvironment. Irreversible electroporation (IRE) is a non-thermal tumor ablation modality that employs high-voltage microsecond pulses to transiently permeabilize cell membranes, ultimately inducing cell death. However, the understanding of IRE’s impact beyond the initiation of focal cell death in tumor tissue remains limited. In this study, we demonstrate that IRE triggers a unique mix of cell death pathways and orchestrates a shift in the local tumor microenvironment driven, in part, by reducing the myeloid-derived suppressor cell (MDSC) and regulatory T cell populations and increasing cytotoxic T lymphocytes and neutrophils. We further show that IRE drives induce cell cycle arrest at the G0/G1 phase in vitro and promote inflammatory cell death pathways consistent with pyroptosis and programmed necrosis in vivo . IRE-treated mice exhibited a substantial extension in progression-free survival. However, within a span of 14 days, the tumor immune cell populations reverted to their pre-treatment composition, which resulted in an attenuation of the systemic immune response targeting contralateral tumors and ultimately resulting in tumor regrowth. Mechanistically, we show that IRE augments IFN- γ signaling, resulting in the up-regulation of the PD-L1 checkpoint in pancreatic cancer cells. Together, these findings shed light on potential mechanisms of tumor regrowth following IRE treatment and offer insights into co-therapeutic targets to improve treatment strategies.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Ablation / Animals / Cancer therapies / Cell cycle / cell cycle arrest / Cell death / Cell Line, Tumor / Cell membranes / Cytotoxicity / Disease Models, Animal / Electroporation / Female / G1 phase / Humans / Immune response / Immunity (Disease) / Immunology / immunomodulation / Inflammation / ire / Laboratory animals / Leukocytes (neutrophilic) / Life expectancy / Lymphocytes T / Metastasis / Mice / Mice, Inbred C57BL / Myeloid-Derived Suppressor Cells - immunology / Pancreatic cancer / Pancreatic Neoplasms - immunology / Pancreatic Neoplasms - pathology / Pancreatic Neoplasms - therapy / PD-L1 protein / Pyroptosis / T-Lymphocytes, Regulatory - immunology / Therapeutic targets / tumor ablation / Tumor microenvironment / Tumor Microenvironment - immunology / tumor recurrence / Tumors