Journal Article

Modification of MWCNTs with Bi2WO6 nanoparticles targeting IL-1β and NLRP3 inflammasome via augmented autophagy

Rahoomi, Zenaa R., Samir, Haney, Swelum, Ayman A., Jabir, Majid S., Ahmed, Duha S.

2024

Overview

This study reports the facile hydrothermal synthesis of pure Bi 2 WO 6 and Bi 2 WO 6 nanocomposite at specific molar ratio 1:2.5 of Bi 2 WO 6 :MWCNTs and elucidates their role in modulating the NLRP3 inflammasome pathway via autophagy induction. Comprehensive characterization techniques, including XRD, Raman, UV.Vis PL,FESEM,EDS and TEM, revealed the successful incorporation of MWCNTs into the Bi 2 WO 6 structures, leading to enhanced crystattlinity, reduced band gap energy (2.4 eV) suppressed charge carrier recombination and mitigated nanoparticles aggregation. Notably, the reduced band gap facikitaed improved visible light harvesting, a crucial attribute for photocatalytic applications. Significantly, the nanocompsoite exhibited a remarkable capacity to augment autophagy in bone marrow-derived macrophages (BMDMs), consequently down-regulating the NLRP3 inflammasom activation and IL-1β secretion upon LPS and ATP stimulation. Immunofluorescence assays unveiled increased co-localization of LC3 and NLRP3, suggestion enhanced targeting of NLRP3 by autophagy. Inhibition of autophagy by 3-MA reversed these effects, confirming the pivotal role of autophagy induction. Furthermore, the nanocomposite attenuated caspase-1 activation and ASC oligomerzation, thereby impeding inflammasome assembly. Collectively, these findings underscore the potential of Bi 2 WO 6 nanocompsite as a multifaceted therapeutic platform, levering its tailored optoelectronic properties and sbility to modulate the NLRP3 infalmmasome via autophagy augmentation. This work covers the way for the development of advanced nanomaterials with tunable functionalities for combating inflammatory disorders and antimicrobial applications.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Autophagy / Autophagy - drug effects / Biology and Life Sciences / Bismuth compounds / Bone marrow / Carbon / Carrier recombination / Caspase 1 - metabolism / Caspase-1 / Caustic soda / Current carriers / Electron microscopes / Energy charge / Energy gap / Engineering and Technology / IL-1β / Immunofluorescence / Inflammasomes / Inflammasomes - metabolism / Inflammatory diseases / Interleukin-1beta - metabolism / Localization / Macrophages / Macrophages - drug effects / Macrophages - metabolism / Medicine and Health Sciences / Membranes / Mice / Morphology / Nanocomposites / Nanocomposites - chemistry / Nanomaterials / Nanoparticles / Nanoparticles - chemistry / Nanotechnology / Nitrates / NLR Family, Pyrin Domain-Containing 3 Protein - metabolism / Optoelectronics / Oxidative stress / Photocatalysis / Physical Sciences / Research and Analysis Methods / Spectrum analysis / Tungstates / Tungsten Compounds - chemistry / Tungsten Compounds - pharmacology