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Fiber-optic control and thermometry of single-cell thermosensation logic
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Fiber-optic control and thermometry of single-cell thermosensation logic
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Journal Article
Fiber-optic control and thermometry of single-cell thermosensation logic
2015
Overview
Thermal activation of transient receptor potential (TRP) cation channels is one of the most striking examples of temperature-controlled processes in cell biology. As the evidence indicating the fundamental role of such processes in thermosensation builds at a fast pace, adequately accurate tools that would allow heat receptor logic behind thermosensation to be examined on a single-cell level are in great demand. Here, we demonstrate a specifically designed fiber-optic probe that enables thermal activation with simultaneous online thermometry of individual cells expressing genetically encoded TRP channels. This probe integrates a fiber-optic tract for the delivery of laser light with a two-wire microwave transmission line. A diamond microcrystal fixed on the fiber tip is heated by laser radiation transmitted through the fiber, providing a local heating of a cell culture, enabling a well-controlled TRP-assisted thermal activation of cells. Online local temperature measurements are performed by using the temperature-dependent frequency shift of optically detected magnetic resonance, induced by coupling the microwave field, delivered by the microwave transmission line, to nitrogen—vacancy centers in the diamond microcrystal. Activation of TRP channels is verified by using genetically encoded fluorescence indicators, visualizing an increase in the calcium flow through activated TRP channels.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 9/10
/ Animals
/ Calcium
/ Fiber Optic Technology - instrumentation
/ Fiber Optic Technology - methods
/ Humanities and Social Sciences
/ Humans
/ Internet
/ Lasers
/ Logic
/ Magnetic Resonance Spectroscopy - instrumentation
/ Magnetic Resonance Spectroscopy - methods
/ Optically detected magnetic resonance
/ Optics
/ Science
/ Single-Cell Analysis - instrumentation
/ Single-Cell Analysis - methods
/ Thermometry - instrumentation
/ Transient Receptor Potential Channels - genetics
/ Transient Receptor Potential Channels - metabolism
