Journal Article

NGF Expression and Elevation in Hip Osteoarthritis Patients with Pain and Central Sensitization

Fukushima, Kensuke, Takahira, Naonobu, Satoh, Masashi, Uchida, Kentaro, Koyama, Tomohisa, Saito, Hiroki, Tsuchiya, Maho, Inoue, Gen, Ohashi, Yoshihisa, Takaso, Masashi

2021

Overview

Osteoarthritis (OA) is a chronic degenerative musculoskeletal disease that causes articular cartilage degeneration and chronic pain. Research into OA animal models suggests that elevated NGF levels in the synovium contribute to pain and central sensitization (CS). However, it is unclear whether synovial NGF contributes to CS in patients with OA. We investigated the association between synovial NGF expression and clinical assessments of pain and CS in hip OA (hOA) patients. We also aimed to identify which cells in the synovium of hOA patients express NGF. Sixty-six patients who received total hip replacement and a diagnosis of hOA were enrolled. We measured NGF mRNA expression in synovial samples obtained from 50 patients using qPCR and analyzed the correlation of NGF expression with the CS inventory (CSI) score and Japanese Orthopaedic Association (JOA) score, a clinical scoring system for OA. To identify the synovial cells expressing NGF, we analyzed NGF mRNA expression in CD14+ and CD14- cells, which represent macrophage-rich and fibroblast-rich fractions, respectively, extracted from 8 patients. To further identify which macrophage subtypes express NGF, we examined NGF mRNA expression in CD14high and CD14low cells sorted from 8 patients. Synovial NGF mRNA expression was negatively correlated with JOA score but positively correlated with CSI score (JOA pain, r=−0.337, P=0.017; CSI score, r=0.358, P=0.011). Significantly greater levels of NGF were observed in CD14- cells compared to CD14+ cells (P=0.036) and in CD14high cells compared to CD14low cells (P=0.008). In conclusion, synovial NGF expression is correlated with the degree of pain and CS in hOA patients. NGF is predominantly expressed in synovial fibroblasts. Further, CD14high synovial macrophages expressed higher levels of NGF. Our results may provide a novel NGF-targeted therapeutic strategy for hOA pain.

• Publisher: Hindawi,John Wiley & Sons, Inc
• Subject: Analysis / Animal models / Arthritis / Biomedical materials / Care and treatment / Cartilage / Cartilage (articular) / Cartilage diseases / CD14 antigen / Central Nervous System Sensitization / Chronic pain / Classification / Complications and side effects / Cysts / Degeneration / Female / Fibroblasts / Fibroblasts - metabolism / Gene expression / Genetic aspects / Growth factors / Health aspects / Hip / Humans / Lipopolysaccharide Receptors - genetics / Lipopolysaccharide Receptors - metabolism / Macrophages / Macrophages - metabolism / Male / Measurement / Middle Aged / Musculoskeletal diseases / Nerve growth factor / Nerve Growth Factor - genetics / Nerve Growth Factor - metabolism / Nonsteroidal anti-inflammatory drugs / Orthopedics / Osteoarthritis / Osteoarthritis, Hip - genetics / Pain / Pain - genetics / Patient outcomes / Patients / Physiological aspects / Risk factors / RNA, Messenger - genetics / RNA, Messenger - metabolism / Sensitization (Psychophysiology) / Synovial Membrane - metabolism / Synovium / Thy-1 Antigens - genetics / Thy-1 Antigens - metabolism / Total hip arthroplasty