Asset Details
Do you wish to reserve the book?
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo
Do you wish to request the book?
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo
2016
Overview
Here we describe “Supernova” series of vector systems that enable single-cell labeling and labeled cell-specific gene manipulation, when introduced by in utero electroporation (IUE) or adeno-associated virus (AAV)-mediated gene delivery. In Supernova, sparse labeling relies on low TRE leakage. In a small population of cells with over-threshold leakage, initial tTA-independent weak expression is enhanced by tTA/TRE-positive feedback along with a site-specific recombination system (e.g., Cre/loxP, Flpe/FRT). Sparse and bright labeling by Supernova with little background enables the visualization of the morphological details of individual neurons in densely packed brain areas such as the cortex and hippocampus, both during development and in adulthood. Sparseness levels are adjustable. Labeled cell-specific gene knockout was accomplished by introducing Cre/loxP-based Supernova vectors into floxed mice. Furthermore, by combining with RNAi, TALEN, and CRISPR/Cas9 technologies, IUE-based Supernova achieved labeled cell-specific gene knockdown and editing/knockout without requiring genetically altered mice. Thus, Supernova system is highly extensible and widely applicable for single-cell analyses in complex organs, such as the mammalian brain.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/19
/ 14/35
/ 38/35
/ 38/89
/ 42/41
/ 631/1647
/ 631/378
/ Animals
/ Clustered Regularly Interspaced Short Palindromic Repeats - genetics
/ CRISPR
/ Feedback
/ Female
/ Gene Knockdown Techniques - methods
/ Gene Knockout Techniques - methods
/ Humanities and Social Sciences
/ Humans
/ Leakage
/ Male
/ Mice
/ Recombination, Genetic - genetics
/ Rodents
/ Science
