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Peptide ES15-1 derived from Haemonchus contortus promotes goat Th17 response by regulating the STAT3/RORγt pathway
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Peptide ES15-1 derived from Haemonchus contortus promotes goat Th17 response by regulating the STAT3/RORγt pathway
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Journal Article
Peptide ES15-1 derived from Haemonchus contortus promotes goat Th17 response by regulating the STAT3/RORγt pathway
2025
Overview
Th17 cells play important roles in anti-infective responses. The 15 kDa excretory/secretory protein of
Haemonchus contortus
(HcES-15) has been identified as a promising immune-protective antigen against
H. contortus
infection capable of up-regulating IL-17, IL-4 and IL-10 production. To obtain the peptides that primarily induce the Th17 immune response, we amplified and expressed the peptides ES15-1, ES15-2 and ES15-3 from HcES-15. In vitro studies demonstrated that ES15-1 stimulated transcriptional activation of the STAT3/RORγt signaling pathway and induced IL-17 production in goat peripheral blood mononuclear cells (PBMCs). In vivo studies, flow cytometric analysis revealed that subcutaneous injection of PLGA-encapsulated ES15-1 peptide (PLGA-ES15-1, 50 μg) significantly enhanced Th17 cell differentiation in the spleens of BALB/c mouse. Consistent with these findings, ELISA quantification demonstrated that ES15-1 treatment significantly increased serum levels of pro-inflammatory cytokine (IL-17, IL-1, IL-6, and TNF-α). In goat immune protection studies, goats (
n
= 6) were subcutaneously immunized with 500 μg of PLGA-ES15-1 on days 0 and 14, followed by infection with
H. contortus
infective third-stage larvae (iL3s) 1 week post-second immunization. ES15-1 significantly enhanced serum levels of pro-inflammatory cytokines (IL-17, IL-1, IL-6, TNF-α). At autopsy, vaccinated goats exhibited 69.0% (
p
< 0.001) reduction of fecal egg counts (FEC) and 50.54% (
p
< 0.05) reduction of worm burdens versus controls. Our findings suggested that peptide ES15-1 enhanced Th17 responses through regulation of the STAT3/RORγt pathway, conferring a certain immune protection against
H. contortus
infection.
Publisher
BioMed Central,Springer Nature B.V,BMC
Subject
/ Antigens
/ Antigens, Helminth - immunology
/ eggs
/ Enzymes
/ ES-15
/ Female
/ Genes
/ Goat Diseases - parasitology
/ Goat Diseases - prevention & control
/ Goats
/ Haemonchiasis - parasitology
/ Haemonchiasis - prevention & control
/ Helminth Proteins - immunology
/ Medicine
/ Mice
/ necropsy
/ Peptides
/ Proteins
/ Sheep
/ STAT3 Transcription Factor - genetics
/ STAT3 Transcription Factor - metabolism
/ Veterinary Medicine/Veterinary Science
/ Virology
