Asset Details
Do you wish to reserve the book?
Nucleosomal occupancy changes locally over key regulatory regions during cell differentiation and reprogramming
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Nucleosomal occupancy changes locally over key regulatory regions during cell differentiation and reprogramming
Do you wish to request the book?
Nucleosomal occupancy changes locally over key regulatory regions during cell differentiation and reprogramming
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Nucleosomal occupancy changes locally over key regulatory regions during cell differentiation and reprogramming
2014
Overview
Chromatin structure determines DNA accessibility. We compare nucleosome occupancy in mouse and human embryonic stem cells (ESCs), induced-pluripotent stem cells (iPSCs) and differentiated cell types using MNase-seq. To address variability inherent in this technique, we developed a bioinformatic approach to identify regions of difference (RoD) in nucleosome occupancy between pluripotent and somatic cells. Surprisingly, most chromatin remains unchanged; a majority of rearrangements appear to affect a single nucleosome. RoDs are enriched at genes and regulatory elements, including enhancers associated with pluripotency and differentiation. RoDs co-localize with binding sites of key developmental regulators, including the reprogramming factors Klf4, Oct4/Sox2 and c-Myc. Nucleosomal landscapes in ESC enhancers are extensively altered, exhibiting lower nucleosome occupancy in pluripotent cells than in somatic cells. Most changes are reset during reprogramming. We conclude that changes in nucleosome occupancy are a hallmark of cell differentiation and reprogramming and likely identify regulatory regions essential for these processes.
Changes in chromatin structure impact gene expression programs by modulating accessibility to the transcription machinery. Here, West
et al
. explore differences in nucleosome occupancy between mammalian pluripotent and somatic cells and uncover regulatory regions likely to play key roles in determining cell identity.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Pub. Group
Subject
/ 64/60
/ Animals
/ Cell Differentiation - genetics
/ Cell Differentiation - physiology
/ Cellular Reprogramming - physiology
/ Embryonic Stem Cells - physiology
/ Humanities and Social Sciences
/ Humans
/ Induced Pluripotent Stem Cells - physiology
/ Mice
/ Science
/ Transcription Factors - genetics
