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A novel major facilitator superfamily transporter in Penicillium digitatum (PdMFS2) is required for prochloraz resistance, conidiation and full virulence
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A novel major facilitator superfamily transporter in Penicillium digitatum (PdMFS2) is required for prochloraz resistance, conidiation and full virulence
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A novel major facilitator superfamily transporter in Penicillium digitatum (PdMFS2) is required for prochloraz resistance, conidiation and full virulence
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Journal Article
A novel major facilitator superfamily transporter in Penicillium digitatum (PdMFS2) is required for prochloraz resistance, conidiation and full virulence
2016
Overview
Objectives
To clone a novel major facilitator superfamily (MFS, a large protein family with diverse physiological functions in all kingdoms) transporter gene,
Pdmfs2,
and characterize its function in
Penicillium digitatum
.
Results
A novel MFS transporter gene,
Pdmfs2
, was isolated from
P. digitatum
. The full-length DNA of
Pdmfs2
had a 1590 bp ORF encoding a full-size MFS transporter with 529 amino acids. In a prochloraz-resistant strain (PdHS-F6),
Pdmfs2
transcript level was up-regulated compared with the prochloraz-sensitive strain (PdHS-E3) and could be induced by 7 μg prochloraz/ml. The deletion of
Pdmfs2
(Δ
Pdmfs2
) in PdHS-F6 led to increased susceptibility to prochloraz and lower EC
50
value (the concentration of prochloraz producing 50 % growth inhibition) compared with the PdHS-F6 or complementation strain (CO
Pdmfs2
). The Δ
Pdmfs2
strain was defective in conidia yield and virulence towards citrus fruits, while the complementation of
Pdmfs2
could restore the phenotypic features to a large extent.
Conclusions
Pdmfs2
is the second MFS transporter gene in
P. digitatum
and is required for prochloraz resistance, conidiation and full virulence.
Publisher
Springer Netherlands,Springer Nature B.V
