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Expression analysis and functional study of honeysuckle MYB transcription factors under drought stress
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Expression analysis and functional study of honeysuckle MYB transcription factors under drought stress
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Journal Article
Expression analysis and functional study of honeysuckle MYB transcription factors under drought stress
2025
Overview
In this study, 356
MYB
transcription factors were identified from the genome of honeysuckle, and combined with transcriptome data analysis, 104 of them were found to respond to drought stress. Through phylogenetic analysis, qRT-PCR analysis and correlation analysis of chemical components, three target genes
LjMYB3
,
LjMYB8
and
LjMYB63
were screened from these 104
MYB
transcription factors and overexpressed in
Arabidopsis thaliana
. The results showed that the drought resistance, total flavonoid content and flavonoid biosynthesis-related gene expression levels of transgenic
Arabidopsis
were higher than those of wild-type
Arabidopsis
.Under drought stress,
MYB
transcription factors can activate the expression of drought resistance-related genes, regulate key genes in the flavonoid synthesis pathway, and promote the accumulation of flavonoids. This study promotes the research on transcriptional regulation of honeysuckle
MYB
transcription factors and flavonoid biosynthesis and lays a foundation for unraveling the drought resistance mechanism in honeysuckle.
Publisher
Nature Publishing Group UK,Nature Portfolio
