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Ergot alkaloid biosynthesis in Aspergillus fumigatus: conversion of chanoclavine-I to chanoclavine-I aldehyde catalyzed by a short-chain alcohol dehydrogenase FgaDH
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Ergot alkaloid biosynthesis in Aspergillus fumigatus: conversion of chanoclavine-I to chanoclavine-I aldehyde catalyzed by a short-chain alcohol dehydrogenase FgaDH
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Ergot alkaloid biosynthesis in Aspergillus fumigatus: conversion of chanoclavine-I to chanoclavine-I aldehyde catalyzed by a short-chain alcohol dehydrogenase FgaDH
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Journal Article
Ergot alkaloid biosynthesis in Aspergillus fumigatus: conversion of chanoclavine-I to chanoclavine-I aldehyde catalyzed by a short-chain alcohol dehydrogenase FgaDH
2010
Overview
Ergot alkaloids are toxins and important pharmaceuticals which are produced biotechnologically on an industrial scale. A putative gene fgaDH has been identified in the biosynthetic gene cluster of fumigaclavine C, an ergot alkaloid of the clavine-type. The deduced gene product FgaDH comprises 261 amino acids with a molecular mass of about 27.8 kDa and contains the conserved motifs of classical short-chain dehydrogenases/reductases (SDRs), but shares no worth mentioning sequence similarity with SDRs and other known proteins. The coding region of fgaDH consisting of two exons was amplified by PCR from a cDNA library of Aspergillus fumigatus, cloned into pQE60 and overexpressed in E. coli. The soluble tetrameric His₆-FgaDH was purified to apparent homogeneity and characterized biochemically. It has been shown that FgaDH catalyzes the oxidation of chanoclavine-I in the presence of NAD⁺ resulting in the formation of chanoclavine-I aldehyde, which was unequivocally identified by NMR and MS analyzes. Therefore, FgaDH functions as a chanoclavine-I dehydrogenase and represents a new group of short-chain dehydrogenases. K M values for chanoclavine-I and NAD⁺ were determined at 0.27 and 1.1 mM, respectively. The turnover number was 0.38 s⁻¹.
Publisher
Berlin/Heidelberg : Springer-Verlag,Springer-Verlag,Springer,Springer Nature B.V
Subject
/ Alcohol Dehydrogenase - genetics
/ Alcohol Dehydrogenase - metabolism
/ Aspergillus fumigatus - enzymology
/ Aspergillus fumigatus - metabolism
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Chromatography, High Pressure Liquid
/ drugs
/ E coli
/ Ecology
/ Enzymes
/ ergot
/ Ergot Alkaloids - biosynthesis
/ exons
/ Fundamental and applied biological sciences. Psychology
/ Fungal Proteins - metabolism
/ Fungi
/ Genes
/ Magnetic Resonance Spectroscopy
/ Mycology
/ nuclear magnetic resonance spectroscopy
/ Peptides
/ proteins
/ Toxins
