Journal Article

Bio-oil production for biodiesel industry by Yarrowia lipolytica from volatile fatty acids in two-stage batch culture

Belo, Isabel, Pereira, Ana S., Miranda, Sílvia M., Lopes, Marlene

2022

Overview

Microbial lipids-derived biodiesel is garnering much attention owing to its potential to substitute diesel fuel. In this study, lipid accumulation by Yarrowia lipolytica from volatile fatty acids (VFAs) was studied in a lab-scale stirred tank bioreactor. In batch cultures, Y. lipolytica NCYC 2904 was able to grow in 18 g·L −1 of VFAs (acetate, propionate, and butyrate), and the addition of a co-substrate (glucose) led to a fivefold improvement in lipid concentration. Furthermore, the two-stage batch culture (growth phase in glucose (1st stage) followed by a lipogenic phase in VFAs (2nd stage)) was the best strategy to obtain the highest lipid content in the cells (37%, w/w), with aeration conditions that kept dissolved oxygen concentration between 40% and 50% of saturation during the lipogenic phase. The estimated fuel properties of biodiesel produced from Y. lipolytica NCYC 2904 lipids are comparable with those of the biodiesel produced from vegetable oils and are in accordance with the international standards (EN 14214 and ASTM D6751). The cultivation strategies herein devised enable a sustainable, eco-friendly, and economical production of microbial lipids, based on feedstocks such as VFAs that can be derived from the acidogenic fermentation of organic wastes. Key points • Addition of glucose to VFAs enhances lipids in Y. lipolytica in batch cultures • Two-stage batch culture — growth in glucose followed by VFAs pulse — rises lipids • Dissolved oxygen of 40–50% of saturation is crucial at the lipogenic phase

• Publisher: Springer Berlin Heidelberg,Springer,Springer Nature B.V
• Subject: acetates / Acetic acid / Aeration / Ascomycota / Bacteriology / Batch Cell Culture Techniques / Batch culture / biodiesel / Biodiesel fuels / Biofuels / Biomedical and Life Sciences / Bioreactors / Biosynthesis / Biotechnological Products and Process Engineering / Biotechnology / butyrates / Cell culture / Cultures and culture media / Diesel / diesel fuel / Diesel fuels / Dissolved oxygen / Fatty acids / Fatty Acids - chemistry / Fatty Acids, Volatile - chemistry / feedstocks / Fermentation / Fungi / Glucose / industry / International standards / Life Sciences / lipid content / Lipids / Methods / Microbial Genetics and Genomics / Microbiology / Microorganisms / Nuclear fuels / Organic wastes / Oxygen / Physiological aspects / Plant Oils / Polyphenols / Production processes / Propionic acid / Saturation / Substrates / Vegetable oils / vegetables / Volatile fatty acids / Yarrowia / Yarrowia lipolytica