Asset Details
Do you wish to reserve the book?
Phylogenetic, evolutionary and structural analysis of Canine Parvovirus (CPV-2) antigenic variants circulating in Colombia
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Phylogenetic, evolutionary and structural analysis of Canine Parvovirus (CPV-2) antigenic variants circulating in Colombia
Do you wish to request the book?
Phylogenetic, evolutionary and structural analysis of Canine Parvovirus (CPV-2) antigenic variants circulating in Colombia
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Phylogenetic, evolutionary and structural analysis of Canine Parvovirus (CPV-2) antigenic variants circulating in Colombia
2020
Overview
Canine parvovirus (CPV-2) is the causative agent of haemorrhagic gastroenteritis in canids. Three antigenic variants—CPV-2a, CPV-2b and CPV-2c—have been described, which are determined by variations at residue 426 of the VP2 capsid protein. In Colombia, the CPV-2a and CPV-2b antigenic variants have previously been reported through partial VP2 sequencing. Mutations at residues Asn428Asp and Ala514Ser of variant CPV-2a were detected, implying the appearance of a possible new CPV-2a variant in Colombia. The purpose of the present study was to characterise the full VP2 capsid protein in samples from Antioquia, Colombia. We conducted a cross-sectional study with 56 stool samples from dogs showing clinical symptoms of parvoviral disease. Following DNA extraction from the samples, VP2 amplification was performed using PCR and positive samples were sequenced. Sequence and phylogenetic analyses were performed by comparison with the VP2 gene sequences of the different CPV-2 worldwide. VP2 was amplified in 51.8% of the analysed samples. Sequencing and sequence alignment showed that 93.1% of the amplified samples belonged to the new CPV-2a antigenic variant previously. Analysing the amino acid sequences revealed that all CPV-2a contain Ala297Asn mutations, which are related to the South America I clade, and the Ala514Ser mutation, which allows characterization as a new CPV-2a sub-variant. The Colombian CPV-2b variant presented Phe267Tyr, Tyr324Ile and Thr440Ala, which are related to the Asia-I clade variants. The CPV-2c was not detected in the samples. In conclusion, two antigenic CPV-2 variants of two geographically distant origins are circulating in Colombia. It is crucial to continue characterising CPV-2 to elucidate the molecular dynamics of the virus and to detect new CPV-2 variants that could be becoming highly prevalent in the region.
Publisher
Universidad Cooperativa de Colombia, Facultad de Ciencias de la Salud, Medicina Veterinaría y Zootecnia, Bucaramanga,Medicina veterinaria y zootecnia,MDPI,Bucaramanga,MDPI AG
Subject
/ Animals
/ Canidae
/ Capsid Proteins - immunology
/ DNA
/ Dogs
/ Female
/ Male
/ Mutation
/ Parvoviridae Infections - epidemiology
/ Parvoviridae Infections - veterinary
/ Parvoviridae Infections - virology
/ Parvovirus, Canine - classification
/ Parvovirus, Canine - genetics
/ Parvovirus, Canine - immunology
/ Parvovirus, Canine - isolation & purification
/ Proteins
/ virus
