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QTL detection and putative candidate gene prediction for leaf rolling under moisture stress condition in wheat
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QTL detection and putative candidate gene prediction for leaf rolling under moisture stress condition in wheat
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QTL detection and putative candidate gene prediction for leaf rolling under moisture stress condition in wheat
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Journal Article
QTL detection and putative candidate gene prediction for leaf rolling under moisture stress condition in wheat
2020
Overview
Leaf rolling is an important mechanism to mitigate the effects of moisture stress in several plant species. In the present study, a set of 92 wheat recombinant inbred lines derived from the cross between NI5439 × HD2012 were used to identify QTLs associated with leaf rolling under moisture stress condition. Linkage map was constructed using Axiom 35 K Breeder’s SNP Array and microsatellite (SSR) markers. A linkage map with 3661 markers comprising 3589 SNP and 72 SSR markers spanning 22,275.01 cM in length across 21 wheat chromosomes was constructed. QTL analysis for leaf rolling trait under moisture stress condition revealed 12 QTLs on chromosomes 1B, 2A, 2B, 2D, 3A, 4A, 4B, 5D, and 6B. A stable QTL
Qlr.nhv-5D.2
was identified on 5D chromosome flanked by SNP marker interval
AX-94892575
–
AX-95124447
(5D:338665301–5D:410952987). Genetic and physical map integration in the confidence intervals of
Qlr.nhv-5D.2
revealed 14 putative candidate genes for drought tolerance which was narrowed down to six genes based on in-silico analysis. Comparative study of leaf rolling genes in rice viz.,
NRL1
,
OsZHD1
,
Roc5
, and
OsHB3
on wheat genome revealed five genes on chromosome 5D. Out of the identified genes,
TraesCS5D02G253100
falls exactly in the QTL
Qlr.nhv-5D.2
interval and showed 96.9% identity with
OsZHD1
. Two genes similar to
OsHB3
viz
. TraesCS5D02G052300
and
TraesCS5D02G385300
exhibiting 85.6% and 91.8% identity; one gene
TraesCS5D02G320600
having 83.9% identity with
Roc5
gene; and one gene
TraesCS5D02G102600
showing 100% identity with
NRL1
gene were also identified, however, these genes are located outside
Qlr.nhv-5D.2
interval. Hence,
TraesCS5D02G253100
could be the best potential candidate gene for leaf rolling and can be utilized for improving drought tolerance in wheat.
