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Characterization of key enzymes involved in triacylglycerol biosynthesis in mycobacteria
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Characterization of key enzymes involved in triacylglycerol biosynthesis in mycobacteria
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Characterization of key enzymes involved in triacylglycerol biosynthesis in mycobacteria
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Journal Article
Characterization of key enzymes involved in triacylglycerol biosynthesis in mycobacteria
2021
Overview
Phosphatidic acid phosphatase (PAP) catalyzes the dephosphorylation of phosphatidic acid (PA) yielding diacylglycerol (DAG), the lipid precursor for triacylglycerol (TAG) biosynthesis. PAP activity has a key role in the regulation of PA flux towards TAG or glycerophospholipid synthesis. In this work we have characterized two
Mycobacterium smegmatis
genes encoding for functional PAP proteins. Disruption of both genes provoked a sharp reduction in de novo TAG biosynthesis in early growth phase cultures under stress conditions. In vivo labeling experiments demonstrated that TAG biosynthesis was restored in the ∆PAP mutant when bacteria reached exponential growth phase, with a concomitant reduction of phospholipid synthesis. In addition, comparative lipidomic analysis showed that the ∆PAP strain had increased levels of odd chain fatty acids esterified into TAGs, suggesting that the absence of PAP activity triggered other rearrangements of lipid metabolism, like phospholipid recycling, in order to maintain the wild type levels of TAG. Finally, the lipid changes observed in the ∆PAP mutant led to defective biofilm formation. Understanding the interaction between TAG synthesis and the lipid composition of mycobacterial cell envelope is a key step to better understand how lipid homeostasis is regulated during
Mycobacterium tuberculosis
infection.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Biofilms
/ Escherichia coli - metabolism
/ Humanities and Social Sciences
/ Lipids
/ Mutants
/ Mutation
/ Mycobacterium smegmatis - enzymology
/ Mycobacterium smegmatis - genetics
/ Mycobacterium smegmatis - metabolism
/ Phosphatidate Phosphatase - genetics
/ Phosphatidate Phosphatase - metabolism
/ Phosphatidic Acids - metabolism
/ Science
