Journal Article

The gut microbial metabolite formate exacerbates colorectal cancer progression

Pozdeev, Vitaly Igorevich, Schmoetten, Maryse, Heinken, Almut, Sauter, Thomas, Koncina, Eric, Meiser, Johannes, Slade, Daniel Joseph, Ternes, Dominik, Martin-Gallausiaux, Camille, Grandmougin, Lea, Gantenbein, Manon, Ginolhac, Aurelien, Pacheco, Maria, Zuegel, Nikolaus, Frachet-Bour, Audrey, Neuberger-Castillo, Lorie, Atatri, Sura, Tsenkova, Mina, Karta, Jessica, Miranda, Paulo, Haan, Serge, Letellier, Elisabeth, Rodriguez, Fabien, Schmitz, Martine, Thiele, Ines, Wilmes, Paul, Delbrouck, Catherine, Nguyen, Tam Thuy Dan, Meyers, Marianne, Gaigneaux, Anthoula, Ferrand, Jean-Yves

2022

Overview

The gut microbiome is a key player in the immunomodulatory and protumorigenic microenvironment during colorectal cancer (CRC), as different gut-derived bacteria can induce tumour growth. However, the crosstalk between the gut microbiome and the host in relation to tumour cell metabolism remains largely unexplored. Here we show that formate, a metabolite produced by the CRC-associated bacterium Fusobacterium nucleatum , promotes CRC development. We describe molecular signatures linking CRC phenotypes with Fusobacterium abundance. Cocultures of F. nucleatum with patient-derived CRC cells display protumorigenic effects, along with a metabolic shift towards increased formate secretion and cancer glutamine metabolism. We further show that microbiome-derived formate drives CRC tumour invasion by triggering AhR signalling, while increasing cancer stemness. Finally, F. nucleatum or formate treatment in mice leads to increased tumour incidence or size, and Th17 cell expansion, which can favour proinflammatory profiles. Moving beyond observational studies, we identify formate as a gut-derived oncometabolite that is relevant for CRC progression. Different gut bacteria have been shown to promote colorectal cancer (CRC) progression. The authors identify formate as an oncometabolite derived from Fusobacterium nucleatum , which promotes CRC formation by increasing cancer stemness.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 14/63 / 38/23 / 38/62 / 45 / 45/100 / 45/77 / 59 / 59/5 / 631/326/41/2533 / 631/443/319 / 631/67/1504/1885 / 631/67/2327 / 64 / 64/60 / 82/58 / 96/31 / Animals / Bacteria / Biomedical and Life Sciences / Cancer / Cell size / Classification / Colorectal cancer / Colorectal Neoplasms - metabolism / Datasets / Formates / Fusobacterium nucleatum / Gastrointestinal Microbiome / Gene expression / Genomes / Helper cells / Humans / Immune response / Immunomodulation / Intestinal microflora / Life Sciences / Lymphocytes T / Metabolism / Metabolites / Metastasis / Mice / Microbiomes / Microenvironments / Patients / Phenotypes / Tumor Microenvironment / Tumors / Virulence