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Japanese encephalitis virus neuropenetrance is driven by mast cell chymase
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Journal Article
Japanese encephalitis virus neuropenetrance is driven by mast cell chymase
2019
Overview
Japanese encephalitis virus (JEV) is a leading cause of viral encephalitis. However, the mechanisms of JEV penetration of the blood-brain-barrier (BBB) remain poorly understood. Mast cells (MCs) are granulated innate immune sentinels located perivascularly, including at the BBB. Here we show that JEV activates MCs, leading to the release of granule-associated proteases in vivo. MC-deficient mice display reduced BBB permeability during JEV infection compared to congenic wild-type (WT) mice, indicating that enhanced vascular leakage in the brain during JEV infection is MC-dependent. Moreover, MCs promoted increased JEV infection in the central nervous system (CNS), enhanced neurological deficits, and reduced survival in vivo. Mechanistically, chymase, a MC-specific protease, enhances JEV-induced breakdown of the BBB and cleavage of tight-junction proteins. Chymase inhibition reversed BBB leakage, reduced brain infection and neurological deficits during JEV infection, and prolonged survival, suggesting chymase is a novel therapeutic target to prevent JEV encephalitis.
How Japanese encephalitis virus (JEV) penetrates the blood-brain barrier (BBB) remains unclear. Here, using a genetic mouse model and a virulent JEV strain, the authors show that perivascular mast cells (MC) mediate JEV neuroinvasion and identify the MC-protease chymase as a potential therapeutic target.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/21
/ 13/51
/ 38/77
/ 64/60
/ Animals
/ Blood-Brain Barrier - immunology
/ Blood-Brain Barrier - virology
/ Central Nervous System - pathology
/ Central Nervous System - virology
/ Chymase
/ Encephalitis Virus, Japanese - pathogenicity
/ Encephalitis, Japanese - immunology
/ Encephalitis, Japanese - metabolism
/ Encephalitis, Japanese - mortality
/ Humanities and Social Sciences
/ Humans
/ Leakage
/ Male
/ Mice
/ Proteins
/ Science
/ Survival
/ Viruses
