Asset Details
Do you wish to reserve the book?
Depiction of the genomic and genetic landscape identifies CCL5 as a protective factor in colorectal neuroendocrine carcinoma
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Depiction of the genomic and genetic landscape identifies CCL5 as a protective factor in colorectal neuroendocrine carcinoma
Do you wish to request the book?
Depiction of the genomic and genetic landscape identifies CCL5 as a protective factor in colorectal neuroendocrine carcinoma
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Depiction of the genomic and genetic landscape identifies CCL5 as a protective factor in colorectal neuroendocrine carcinoma
2021
Overview
Background
Colorectal neuroendocrine carcinomas (CRNECs) are highly aggressive tumours with poor prognosis and low incidence. To date, the genomic landscape and molecular pathway alterations have not been elucidated.
Methods
Tissue sections and clinical information of CRNEC (
n
= 35) and CR neuroendocrine tumours (CRNETs) (
n
= 25) were collected as an in-house cohort (2010–2020). Comprehensive genomic and expression panels (AmoyDx® Master Panel) were applied to identify the genomic and genetic alterations of CRNEC. Through the depiction of the genomic landscape and transcriptome profile, we compared the difference between CRNEC and CRNET. Reverse transcription-polymerase chain reaction and immunofluorescence staining were performed to confirm the genetic alterations.
Results
High tumour mutation load was observed in CRNEC compared with CRNET. CRNECs showed a “cold” immune landscape and increased endothelial cell activity compared with NETs. Importantly, PAX5 was aberrantly expressed in CRNEC and predicted a poor prognosis of CRNECs. CCL5, a factor that is considered an immunosuppressive factor in several tumour types, was strongly expressed in CRNEC patients with long-term survival and correlated with high CD8
+
T cell infiltration.
Conclusion
Through the depiction of the genomic landscape and transcriptome profile, we demonstrated alterations in molecular pathways and potential targets for immunotherapy in CRNEC.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Adult
/ Aged
/ Biomedical and Life Sciences
/ Carcinoma, Neuroendocrine - genetics
/ Carcinoma, Neuroendocrine - immunology
/ CD8-Positive T-Lymphocytes - immunology
/ Colorectal Neoplasms - genetics
/ Colorectal Neoplasms - immunology
/ Female
/ Gene Expression Profiling - methods
/ Gene Expression Regulation, Neoplastic
/ Genomics
/ Humans
/ Male
/ Mutation
/ Neuroendocrine Tumors - genetics
/ Neuroendocrine Tumors - immunology
/ Oncology
/ Tumors
