Asset Details
Do you wish to reserve the book?
Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation
Do you wish to request the book?
Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation
2022
Overview
Spermatogenesis is a complex biological process that produces haploid spermatozoa and requires precise regulation by many tissue-specific factors. In this study, we explored the role and mechanism of Fork head box J2 (FOXJ2, which is highly expressed in spermatocytes) in the regulation of spermatogenesis using a germline-specific conditional
Foxj2
knock-in mouse model (
Stra8-Cre; Foxj2
tg/tg
mouse).
Foxj2
overexpression in mouse testes led to spermatogenesis failure, which started at the initiation of meiosis, and resulted in male infertility. Lysosomes and autophagy-related genes were upregulated in
Stra8-cre; Foxj2
tg/tg
mouse testes and the number of autolysosomes in the spermatocytes in
Stra8-cre; Foxj2
tg/tg
mice was increased. Chromatin immunoprecipitation-PCR and Dual-luciferase reporter assays showed that
Lamp2
(encoding lysosome‐associated membrane protein‐2) was a target of FOXJ2.
Foxj2
overexpression increased the expression levels of
Lamp2a
and
Hsc70
(70-kDa cytoplasmic heat shock protein) in the
Stra8-cre; Foxj2
tg/tg
mouse testes. Our results suggested that
Foxj2
overexpression in the germ cells of mouse testes affects chaperone-mediated autophagy by upregulating LAMP2A, leading to spermatogenesis failure at the initiation of meiosis, thus resulting in male infertility. Our findings provide a new insight into the function of FOXJ2 in spermatogenesis and the significance of autophagy regulation in spermatogenesis.
Publisher
Nature Publishing Group UK,Springer Nature B.V,Nature Publishing Group
Subject
/ 13/44
/ 13/51
/ 14/28
/ 38/90
/ 38/91
/ 45/77
/ 64/60
/ Animals
/ Biomedical and Life Sciences
/ Forkhead Transcription Factors - genetics
/ Forkhead Transcription Factors - metabolism
/ Humans
/ Infertility, Male - genetics
/ Infertility, Male - metabolism
/ Lysosomal-Associated Membrane Protein 2 - metabolism
/ Male
/ Meiosis
/ Mice
/ Testes
