Journal Article

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia

Day, Matthew, Tarchini, Basile, Jarysta, Amandine, Beirl, Alisha, Kindt, Katie S., Flonard, Michaela, Akturk, Anil

2021

Overview

Hair cells detect sound, head position or water movements when their mechanosensory hair bundle is deflected. Each hair bundle has an asymmetric architecture that restricts stimulus detection to a single axis. Coordinated hair cell orientations within sensory epithelia further tune stimulus detection at the organ level. Here, we identify GPR156, an orphan GPCR of unknown function, as a critical regulator of hair cell orientation. We demonstrate that the transcription factor EMX2 polarizes GPR156 distribution, enabling it to signal through Gαi and trigger a 180° reversal in hair cell orientation. GPR156-Gαi mediated reversal is essential to establish hair cells with mirror-image orientations in mouse otolith organs in the vestibular system and in zebrafish lateral line. Remarkably, GPR156-Gαi also instructs hair cell reversal in the auditory epithelium, despite a lack of mirror-image organization. Overall, our work demonstrates that conserved GPR156-Gαi signaling is integral to the framework that builds directional responses into mechanosensory epithelia. Sensory hair cells develop an asymmetric architecture to restrict stimulus detection to a single axis. Here the authors identify GPR156 as directing a 180-degree reversal in hair cell orientation through Gαi, downstream of EMX2 in the mouse inner ear and zebrafish lateral line.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/1 / 13/106 / 13/51 / 14 / 14/19 / 14/28 / 38 / 38/39 / 631/136/1660 / 631/208/135 / 631/378/2619/1387 / 631/378/3917 / 631/80/85 / 64/60 / Animals / Asymmetry / Cell Polarity - genetics / Danio rerio / Epithelium / Epithelium - metabolism / Female / G protein-coupled receptors / GTP-Binding Protein alpha Subunits, Gi-Go - genetics / GTP-Binding Protein alpha Subunits, Gi-Go - metabolism / Hair / Hair cells / Hair Cells, Auditory - cytology / Hair Cells, Auditory - metabolism / Hearing protection / Homeodomain Proteins - genetics / Homeodomain Proteins - metabolism / Humanities and Social Sciences / Inner ear / Lateral line / Male / Mice / Mice, Inbred C57BL / Mice, Knockout / Mice, Transgenic / Microscopy, Confocal - methods / multidisciplinary / Orientation / Orientation behavior / Otolith organs / Receptors, G-Protein-Coupled - genetics / Receptors, G-Protein-Coupled - metabolism / Science / Science (multidisciplinary) / Transcription Factors - genetics / Transcription Factors - metabolism / Vestibular system / Zebrafish / Zebrafish - metabolism