Asset Details
Do you wish to reserve the book?
Embryo model completes gastrulation to neurulation and organogenesis
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Embryo model completes gastrulation to neurulation and organogenesis
Do you wish to request the book?
Embryo model completes gastrulation to neurulation and organogenesis
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Embryo model completes gastrulation to neurulation and organogenesis
2022
Overview
Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in vitro
1
–
5
, but their developmental potential is substantially extended by interactions with extraembryonic stem cells, including trophoblast stem (TS) cells, extraembryonic endoderm stem (XEN) cells and inducible XEN (iXEN) cells
6
–
11
. Here we assembled stem cell-derived embryos in vitro from mouse ES cells, TS cells and iXEN cells and showed that they recapitulate the development of whole natural mouse embryo in utero up to day 8.5 post-fertilization. Our embryo model displays headfolds with defined forebrain and midbrain regions and develops a beating heart-like structure, a trunk comprising a neural tube and somites, a tail bud containing neuromesodermal progenitors, a gut tube, and primordial germ cells. This complete embryo model develops within an extraembryonic yolk sac that initiates blood island development. Notably, we demonstrate that the neurulating embryo model assembled from
Pax6
-knockout ES cells aggregated with wild-type TS cells and iXEN cells recapitulates the ventral domain expansion of the neural tube that occurs in natural, ubiquitous
Pax6
-knockout embryos. Thus, these complete embryoids are a powerful in vitro model for dissecting the roles of diverse cell lineages and genes in development. Our results demonstrate the self-organization ability of ES cells and two types of extraembryonic stem cells to reconstitute mammalian development through and beyond gastrulation to neurulation and early organogenesis.
Synthetic mouse embryos assembled from embryonic stem cells, trophoblast stem cells and induced extraembryonic endoderm stem cells closely recapitulate the development of wild-type and mutant natural mouse embryos up to embryonic day 8.5.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/1
/ 13/100
/ 13/106
/ 13/109
/ 13/51
/ 14/1
/ 14/19
/ 14/32
/ 14/63
/ Animals
/ Chorion
/ Cytology
/ Embryo, Mammalian - cytology
/ Embryo, Mammalian - embryology
/ Embryonic Stem Cells - cytology
/ Embryos
/ Humanities and Social Sciences
/ Mice
/ PAX6 Transcription Factor - deficiency
/ PAX6 Transcription Factor - genetics
/ Science
/ Yolk sac
