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Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli
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Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli
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Journal Article
Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli
2021
Overview
Genetically programmed circuits allowing bifunctional dynamic regulation of enzyme expression have far-reaching significances for various bio-manufactural purposes. However, building a bio-switch with a post log-phase response and reversibility during scale-up bioprocesses is still a challenge in metabolic engineering due to the lack of robustness. Here, we report a robust thermosensitive bio-switch that enables stringent bidirectional control of gene expression over time and levels in living cells. Based on the bio-switch, we obtain tree ring-like colonies with spatially distributed patterns and transformer cells shifting among spherical-, rod- and fiber-shapes of the engineered
Escherichia coli
. Moreover, fed-batch fermentations of recombinant
E. coli
are conducted to obtain ordered assembly of tailor-made biopolymers polyhydroxyalkanoates including diblock- and random-copolymer, composed of 3-hydroxybutyrate and 4-hydroxybutyrate with controllable monomer molar fraction. This study demonstrates the possibility of well-organized, chemosynthesis-like block polymerization on a molecular scale by reprogrammed microbes, exemplifying the versatility of thermo-response control for various practical uses.
Genetic circuits can be built with bifunctional dynamic regulation of gene expression. Here the authors design a thermosensitive switch for spatial and temporal control of colony pattern, cell shape and polymer production.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 3-Hydroxybutyric Acid - metabolism
/ 38/62
/ 45/91
/ Circuits
/ Colonies
/ E coli
/ Escherichia coli - growth & development
/ Escherichia coli - metabolism
/ Gene Expression Regulation, Bacterial
/ Green Fluorescent Proteins - genetics
/ Green Fluorescent Proteins - metabolism
/ Humanities and Social Sciences
/ Hydroxybutyrates - metabolism
/ Luminescent Proteins - genetics
/ Luminescent Proteins - metabolism
/ Metabolic Engineering - methods
/ Microorganisms, Genetically-Modified
/ Polyhydroxyalkanoates - metabolism
/ Polymers
/ Science
