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Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters
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Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters
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Journal Article
Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters
2021
Overview
Norepinephrine is a biogenic amine neurotransmitter that has widespread effects on alertness, arousal and pain sensation. Consequently, blockers of norepinephrine uptake have served as vital tools to treat depression and chronic pain. Here, we employ the
Drosophila melanogaster
dopamine transporter as a surrogate for the norepinephrine transporter and determine X-ray structures of the transporter in its substrate-free and norepinephrine-bound forms. We also report structures of the transporter in complex with inhibitors of chronic pain including duloxetine, milnacipran and a synthetic opioid, tramadol. When compared to dopamine, we observe that norepinephrine binds in a different pose, in the vicinity of subsite C within the primary binding site. Our experiments reveal that this region is the binding site for chronic pain inhibitors and a determinant for norepinephrine-specific reuptake inhibition, thereby providing a paradigm for the design of specific inhibitors for catecholamine neurotransmitter transporters.
The
Drosophila
dopamine transporter (dDAT) is a catecholamine neurotransmitter transporter that resembles the human norepinephrine transporter (hNET). Here the authors report X-ray structures of the dDAT in substrate-free form, norepinephrine-bound form and dDAT bound to commonly prescribed chronic pain inhibitors duloxetine, milnacipran and tramadol and shed light on the structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/106
/ 82/80
/ 82/83
/ Analgesics, Opioid - chemistry
/ Animals
/ Arousal
/ Dopamine
/ Dopamine Plasma Membrane Transport Proteins
/ Drosophila Proteins - chemistry
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Humanities and Social Sciences
/ Humans
/ Insects
/ Mice
/ Neurotransmitter Transport Proteins - chemistry
/ Neurotransmitter Transport Proteins - genetics
/ Neurotransmitter Transport Proteins - metabolism
/ Opioids
/ Pain
/ Science
/ Tramadol
