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DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability
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DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability
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Journal Article
DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability
2020
Overview
The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. The reason for the emergence and strict requirement of the additional initiation factor Bdp1 in the RNA polymerase (RNAP) III system, however, remained elusive. A poorly studied aspect in this context is the effect of DNA strain arising from DNA compaction and transcriptional activity on initiation complex formation. We made use of a DNA origami-based force clamp to follow the assembly of human initiation complexes in the RNAP II and RNAP III systems at the single-molecule level under piconewton forces. We demonstrate that TBP-DNA complexes are force-sensitive and TFIIB is sufficient to stabilise TBP on a strained promoter. In contrast, Bdp1 is the pivotal component that ensures stable anchoring of initiation factors, and thus the polymerase itself, in the RNAP III system. Thereby, we offer an explanation for the crucial role of Bdp1 for the high transcriptional output of RNAP III.
TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. Here, the authors use a DNA origami-based force clamp to investigate the assembly dynamics of human initiation complexes in the RNAP II and RNAP III systems at the single-molecule level under pico newton forces.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 631/45
/ 631/57
/ Assembly
/ DNA
/ DNA, Single-Stranded - chemistry
/ DNA, Single-Stranded - metabolism
/ DNA, Single-Stranded - ultrastructure
/ Fluorescence Resonance Energy Transfer
/ Humanities and Social Sciences
/ Kinetics
/ Microscopy, Electron, Transmission
/ Molecular Probes - chemistry
/ Molecular Probes - metabolism
/ Molecular Probes - ultrastructure
/ Proteins
/ Recombinant Proteins - metabolism
/ RNA
/ RNA Polymerase III - chemistry
/ RNA Polymerase III - metabolism
/ Science
/ Single Molecule Imaging - methods
/ TATA-Box Binding Protein - metabolism
/ Transcription Factor TFIIIB - metabolism
