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Functionally overlapping intra- and extralysosomal pathways promote bis(monoacylglycero)phosphate synthesis in mammalian cells
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Functionally overlapping intra- and extralysosomal pathways promote bis(monoacylglycero)phosphate synthesis in mammalian cells
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Functionally overlapping intra- and extralysosomal pathways promote bis(monoacylglycero)phosphate synthesis in mammalian cells
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Journal Article
Functionally overlapping intra- and extralysosomal pathways promote bis(monoacylglycero)phosphate synthesis in mammalian cells
2024
Overview
Bis(monoacylglycero)phosphate (BMP) is a major phospholipid constituent of intralumenal membranes in late endosomes/lysosomes, where it regulates the degradation and sorting of lipid cargo. Recent observations suggest that the Batten disease-associated protein CLN5 functions as lysosomal BMP synthase. Here, we show that transacylation reactions catalyzed by cytosolic and secreted enzymes enhance BMP synthesis independently of CLN5. The transacylases identified in this study are capable of acylating the precursor lipid phosphatidylglycerol (PG), generating acyl-PG, which is subsequently hydrolyzed to BMP. Extracellularly, acyl-PG and BMP are generated by endothelial lipase in cooperation with other serum enzymes of the pancreatic lipase family. The intracellular acylation of PG is catalyzed by several members of the cytosolic phospholipase A2 group IV (PLA2G4) family. Overexpression of secreted or cytosolic transacylases was sufficient to correct BMP deficiency in HEK293 cells lacking
CLN5
. Collectively, our observations suggest that functionally overlapping pathways promote BMP synthesis in mammalian cells.
Bis(monoacylglycero)phosphate (BMP) is an important component of late endosomal and lysosomal membranes. In this study, the authors show that both intra- and extralysosomal pathways can contribute to BMP synthesis.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/44
/ 14/34
/ 45/70
/ 64/60
/ 82/58
/ 82/80
/ 82/83
/ Animals
/ Enzymes
/ Humanities and Social Sciences
/ Humans
/ Lipase
/ Lipids
/ Lysophospholipids - metabolism
/ Mammals
/ Mice
/ Neuronal ceroid lipofuscinosis
/ Phosphatidylglycerols - metabolism
/ Science
