Asset Details
Do you wish to reserve the book?
Molecular genetic analysis of colorectal carcinoma with an aggressive extraintestinal immunohistochemical phenotype
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Molecular genetic analysis of colorectal carcinoma with an aggressive extraintestinal immunohistochemical phenotype
Do you wish to request the book?
Molecular genetic analysis of colorectal carcinoma with an aggressive extraintestinal immunohistochemical phenotype
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Molecular genetic analysis of colorectal carcinoma with an aggressive extraintestinal immunohistochemical phenotype
2024
Overview
Colorectal cancer (CRC) is a leading global cause of illness and death. There is a need for identification of better prognostic markers beyond traditional clinical variables like grade and stage. Previous research revealed that abnormal expression of cytokeratin 7 (CK7) and loss of the intestinal-specific Special AT-rich sequence-binding protein 2 (SATB2) are linked to poor CRC prognosis. This study aimed to explore these markers’ prognostic significance alongside two extraintestinal mucins (MUC5AC, MUC6), claudin 18, and MUC4 in 285 CRC cases using immunohistochemistry on tissue microarrays (TMAs). CK7 expression and SATB2-loss were associated with MUC5AC, MUC6, and claudin 18 positivity. These findings suggest a distinct \"non-intestinal\" immunohistochemical profile in CRC, often right-sided, SATB2-low, with atypical expression of CK7 and non-colorectal mucins (MUC5AC, MUC6). Strong MUC4 expression negatively impacted cancer-specific survival (hazard ratio = 2.7, p = 0.044). Genetic analysis via next-generation sequencing (NGS) in CK7 + CRCs and those with high MUC4 expression revealed prevalent mutations in
TP53, APC, BRAF, KRAS, PIK3CA, FBXW7,
and
SMAD4,
consistent with known CRC mutation patterns. NGS also identified druggable variants in
BRAF, PIK3CA,
and
KRAS
. CK7 + tumors showed intriguingly common (31.6%)
BRAF
V600E mutations corelating with poor prognosis, compared to the frequency described in the literature and databases. Further research on larger cohorts with a non-colorectal immunophenotype and high MUC4 expression is needed.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/4020
/ 692/4028
/ 692/53
/ Adult
/ Aged
/ Biomarkers, Tumor - genetics
/ Biomarkers, Tumor - metabolism
/ Class I Phosphatidylinositol 3-Kinases - genetics
/ Class I Phosphatidylinositol 3-Kinases - metabolism
/ Colorectal Neoplasms - genetics
/ Colorectal Neoplasms - metabolism
/ Colorectal Neoplasms - mortality
/ Colorectal Neoplasms - pathology
/ Female
/ High-Throughput Nucleotide Sequencing
/ Humanities and Social Sciences
/ Humans
/ Male
/ Matrix Attachment Region Binding Proteins - genetics
/ Matrix Attachment Region Binding Proteins - metabolism
/ Mucin
/ Mucins
/ Mutation
/ NGS
/ Proto-Oncogene Proteins B-raf - genetics
/ Proto-Oncogene Proteins p21(ras) - genetics
/ SATB2
/ Science
