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Isolation and characterization of DNA aptamers against the HlyE antigen of Salmonella Typhi
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Isolation and characterization of DNA aptamers against the HlyE antigen of Salmonella Typhi
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Journal Article
Isolation and characterization of DNA aptamers against the HlyE antigen of Salmonella Typhi
2024
Overview
Aptamers have emerged as prominent ligands in clinical diagnostics because they provide various advantages over antibodies, such as quicker generation time, reduced manufacturing costs, minimal batch-to-batch variability, greater modifiability, and improved thermal stability. In the present study, we isolated and characterized DNA aptamers that can specifically bind to the hemolysin E (HlyE) antigen of
Salmonella
Typhi for future development of typhoid diagnostic tests. The DNA aptamers against
Salmonella
Typhi HlyE were isolated using systematic evolution of ligands by exponential enrichment (SELEX), and their binding affinity and specificity were assessed utilizing enzyme-linked oligonucleotide assay (ELONA). A total of 11 distinct aptamers were identified, and the binding affinities and species selectivities of the three most probable aptamers were determined. Kd values were obtained in the nanomolar range, with the highest affinity of 83.6 nM determined for AptHlyE97. In addition, AptHlyE11, AptHlyE45 and AptHlyE97 clearly distinguished
S.
Typhi HlyE from other tested bacteria, such as
Salmonella
Paratyphi A,
Salmonella
Paratyphi B,
Shigella flexneri
,
Klebsiella pneumonia
and
Escherichia coli
, therefore displaying desirable specificity. These novel aptamers could be used as diagnostic ligands for the future development of inexpensive and effective point-of-care tests for typhoid surveillance, especially in developing countries of the tropics and subtropics.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 631/326
/ 631/337
/ 631/61
/ 692/4017
/ Affinity
/ Antigens
/ Antigens, Bacterial - immunology
/ Antigens, Bacterial - isolation & purification
/ Aptamers
/ Aptamers, Nucleotide - chemistry
/ DNA
/ E coli
/ Humanities and Social Sciences
/ Humans
/ LDCs
/ Ligands
/ Science
/ SELEX
/ SELEX Aptamer Technique - methods
/ Typhoid
