Journal Article

A YAP-centered mechanotransduction loop drives collective breast cancer cell invasion

Fodde, Riccardo, Roukens, M. Guy, Smits, Daan, Jansen, Karin A., Enserink, Lotte, Khalil, Antoine A., van der Net, Mirjam, Visser, Daan, Westland, Denise, Derksen, Patrick WB, van Zwieten, Kitty, Pasolli, Milena, Haughton, Peter D., Park, Morag, Tavares, Sandra, Moelans, Cathy B., Gloerich, Martijn, van Diest, Paul J., Zwartkruis, Fried. J. T., Tak, Max, Verhagen, Mathijs P., de Rooij, Johan, Jansen, Lisa, Koorman, Thijs, Fortier, Anne-Marie, El-Gammal, Abdelrahman G.

2024

Overview

Dense and aligned Collagen I fibers are associated with collective cancer invasion led by protrusive tumor cells, leader cells. In some breast tumors, a population of cancer cells (basal-like cells) maintain several epithelial characteristics and express the myoepithelial/basal cell marker Keratin 14 (K14). Emergence of leader cells and K14 expression are regarded as interconnected events triggered by Collagen I, however the underlying mechanisms remain unknown. Using breast carcinoma organoids, we show that Collagen I drives a force-dependent loop, specifically in basal-like cancer cells. The feed-forward loop is centered around the mechanotransducer Yap and independent of K14 expression. Yap promotes a transcriptional program that enhances Collagen I alignment and tension, which further activates Yap. Active Yap is detected in invading breast cancer cells in patients and required for collective invasion in 3D Collagen I and in the mammary fat pad of mice. Our work uncovers an essential function for Yap in leader cell selection during collective cancer invasion. Aligned collagen I is associated with the emergence of leader cells that are responsible for collective invasion. Here, the authors show that Collagen I and Yap signalling are in a feed-forward loop to drive the collective invasion of basal-like tumour cells.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13 / 13/106 / 13/109 / 13/51 / 13/89 / 13/95 / 14 / 14/19 / 14/34 / 59 / 631/67/327 / 631/80/84/2334 / 631/80/84/2336 / 64 / 64/60 / 96 / Adaptor Proteins, Signal Transducing - genetics / Adaptor Proteins, Signal Transducing - metabolism / Animals / Breast cancer / Breast carcinoma / Breast Neoplasms - genetics / Breast Neoplasms - metabolism / Breast Neoplasms - pathology / Cell Line, Tumor / Collagen / Collagen (type I) / Collagen Type I - metabolism / Female / Fibers / Gene Expression Regulation, Neoplastic / Humanities and Social Sciences / Humans / Keratin / Mechanotransduction / Mechanotransduction, Cellular / Mice / multidisciplinary / Neoplasm Invasiveness / Organoids / Organoids - metabolism / Organoids - pathology / Science / Science (multidisciplinary) / Transcription Factors - genetics / Transcription Factors - metabolism / Tumor cells / Tumors / YAP-Signaling Proteins - metabolism / Yes-associated protein