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5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression
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5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression
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Journal Article
5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression
2023
Overview
DNA methylation at the fifth position of cytosine (5-methylcytosine, 5mC) is a crucial epigenetic modification for regulating gene expression, but little is known about how it regulates gene expression in insects. Here, we pursue the detailed molecular mechanism by which DNMT1-mediated 5mC maintenance regulates female reproduction in the German cockroach,
Blattella germanica
. Our results show that
Dnmt1
knockdown decreases the level of 5mC in the ovary, upregulating numerous genes during choriogenesis, especially the transcription factor
ftz-f1
. The hypomethylation at the
ftz-f1
promoter region increases and prolongs
ftz-f1
expression in ovarian follicle cells during choriogenesis, which consequently causes aberrantly high levels of 20-hydroxyecdysone and excessively upregulates the extracellular matrix remodeling gene
Mmp1
. These changes further impair choriogenesis and disrupt fertilization by causing anoikis of the follicle cells, a shortage of chorion proteins, and malformation of the sponge-like bodies. This study significantly advances our understanding of how DNA 5mC modification regulates female reproduction in insects.
The mechanism by which DNA methylation regulates female reproduction in insects is largely unknown. Here Zhao
et al
. demonstrate that the 5mC modification orchestrates timely choriogenesis and proper fertilization by preventing prolonged ftz-f1 expression in the German cockroach.
