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Mouse MRE11-RAD50-NBS1 is needed to start and extend meiotic DNA end resection
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Mouse MRE11-RAD50-NBS1 is needed to start and extend meiotic DNA end resection
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Journal Article
Mouse MRE11-RAD50-NBS1 is needed to start and extend meiotic DNA end resection
2025
Overview
Nucleolytic resection of DNA ends is critical for homologous recombination, but its mechanism is not fully understood, particularly in mammalian meiosis. Here we examine roles of the conserved MRN complex (MRE11, RAD50, and NBS1) through genome-wide analysis of meiotic resection during spermatogenesis in mice with various MRN mutations, including several that cause chromosomal instability in humans. Meiotic DSBs form at elevated levels but remain unresected if
Mre11
is conditionally deleted, thus MRN is required for both resection initiation and regulation of DSB numbers. Resection lengths are reduced to varying degrees in MRN hypomorphs or if MRE11 nuclease activity is attenuated in a conditional nuclease-dead
Mre11
model. These findings unexpectedly establish that MRN is needed for longer-range extension of resection beyond that carried out by the orthologous proteins in budding yeast meiosis. Finally, resection defects are additively worsened by combining MRN and
Exo1
mutations, and mice that are unable to initiate resection or have greatly curtailed resection lengths experience catastrophic spermatogenic failure. Our results elucidate MRN roles in meiotic DSB end processing and establish the importance of resection for mammalian meiosis.
The DNA breaks that initiate recombination during meiosis are resected by nucleases, but the mechanism of this resection in mammals is poorly understood. Here, the authors examine the roles of the MRE11-RAD50- NBS1 complex in beginning and extending resection during meiosis in male mice.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/51
/ 14/63
/ 45/15
/ 45/23
/ 64/60
/ Animals
/ Cell Cycle Proteins - genetics
/ Cell Cycle Proteins - metabolism
/ DNA
/ DNA Repair Enzymes - genetics
/ DNA Repair Enzymes - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Exodeoxyribonucleases - genetics
/ Exodeoxyribonucleases - metabolism
/ Humanities and Social Sciences
/ Male
/ Mammals
/ Meiosis
/ Mice
/ MRE11 Homologue Protein - genetics
/ MRE11 Homologue Protein - metabolism
/ Mutation
/ Nuclear Proteins - metabolism
/ Nuclease
/ Science
/ Yeast
