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TALE-mediated modulation of transcriptional enhancers in vivo
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TALE-mediated modulation of transcriptional enhancers in vivo
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Journal Article
TALE-mediated modulation of transcriptional enhancers in vivo
2013
Overview
Transcription activator–like effectors are used for
in vivo
activation and repression of endogenous promoters and enhancers in the fruit fly.
We tested whether transcription activator–like effectors (TALEs) could mediate repression and activation of endogenous enhancers in the
Drosophila
genome. TALE repressors (TALERs) targeting each of the five
even-skipped
(
eve
) stripe enhancers generated repression specifically of the focal stripes. TALE activators (TALEAs) targeting the
eve
promoter or enhancers caused increased expression primarily in cells normally activated by the promoter or targeted enhancer, respectively. This effect supports the view that repression acts in a dominant fashion on transcriptional activators and that the activity state of an enhancer influences TALE binding or the ability of the VP16 domain to enhance transcription. In these assays, the Hairy repression domain did not exhibit previously described long-range transcriptional repression activity. The phenotypic effects of TALER and TALEA expression in larvae and adults are consistent with the observed modulations of
eve
expression. TALEs thus provide a novel tool for detection and functional modulation of transcriptional enhancers in their native genomic context.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Analysis
/ Animals
/ Biomedical Engineering/Biotechnology
/ DNA
/ Drosophila melanogaster - genetics
/ Drosophila Proteins - genetics
/ Female
/ Gene Expression Regulation, Developmental
/ Genomes
/ Genomics
/ Homeodomain Proteins - genetics
/ Insects
/ Larvae
/ Male
/ Organisms, Genetically Modified
/ Proteins
