Journal Article

The Evaluation of a New ELISA-Based Kit for Total Microcystins as an Early Detection Tool for Microcystin Blooms in Source Waters and Its Application State-Wide to Oregon Source and Finished Drinking Waters

Pepich, Barry V., Borisenko, Aaron, Clauson, Kale, Adams, William A., McBride, Theresa, Adams, Katie, Dyer, Stuart W., Labiosa, Rochelle G., Fairchild, Ned

2025

Overview

Due to cyanobacterial toxin (cyanotoxin) contamination issues in 2018, the city of Salem, Oregon, issued a 33-day do-not-drink advisory for vulnerable people among the 200,000 residents. After the incident, the state of Oregon put in place drinking water rules to require the routine testing of raw water, as well as finished water, in cases where the raw water cyanotoxin concentrations exceeded trigger values. The United States Environmental Protection Agency (EPA) total microcystins drinking water health advisory level (HAL) for small children is 0.3 µg/L. This is equivalent to the minimum reporting level (MRL) for EPA Method 546. Consequently, there was no ability to provide early warnings via toxin testing for total microcystins using the EPA method. In this study, we performed a comparison of the precision and accuracy of the enzyme-linked immunosorbent assay (ELISA) described in the EPA method to a more sensitive assay, the Streptavidin-enhanced Sensitivity (SAES) assay. Based on these precision and accuracy studies and quantitation limit determinations and confirmations, the EPA Office of Ground Water and Drinking Water (OGWDW) has concluded the SAES kit meets the requirements of EPA Method 546. With an MRL that is one-third of the original concentration, the new kit provides a small but critical window for identifying early warnings. Challenges remain with providing early warnings due to the variability in bloom dynamics; however, the new MRL allowed Oregon to lower the trigger level for susceptible systems, thereby providing an additional early warning.

• Publisher: MDPI AG,MDPI
• Subject: Algae / Assaying / Automation / Contamination / Cyanobacteria / Drinking water / Drinking Water - analysis / Drinking Water - microbiology / ELISA / Environmental aspects / Environmental management / Environmental Monitoring - methods / Environmental protection / Enzyme-linked immunosorbent assay / Enzyme-Linked Immunosorbent Assay - methods / Enzymes / Groundwater / Humans / Identification and classification / Medical research / microcystin / Microcystins / Microcystins - analysis / Microcystis / Oregon / Public health / Raw water / reference method / Streptavidin / Testing / Toxicity / Toxins / Validation studies / Water Microbiology / Water quality