Journal Article

High Prevalence of Rare Monogenic Forms of Obesity in Obese Guadeloupean Afro-Caribbean Children

Billy, Rachel, Lacorte, Jean-Marc, De Graeve, Franck, Michel, Carl-Thony, Sand, Olivier, Bonnefond, Amélie, Dhennin, Véronique, Larifla, Laurent, Rabearivelo, Iandry, Durand, Emmanuelle, Foucan, Lydia, Armand, Christophe, Rambhojan, Christine, Froguel, Philippe

2018

Overview

Abstract Context The population of Guadeloupe Island exhibits a high prevalence of obesity. Objective We aimed to investigate whether rare genetic mutations in genes involved in monogenic obesity (or diabetes) might be causal in this population of Afro-Caribbean ancestry. Design and Setting This was a secondary analysis of a study on obesity conducted in schoolchildren from Guadeloupe in 2013 that aimed to assess changes in children’s profiles after a lifestyle intervention program. Through next-generation sequencing, we sequenced coding regions of 59 genes involved in monogenic obesity or diabetes in participants from this study. Participants and Interventions A total of 25 obese schoolchildren from Guadeloupe were screened for rare mutations (nonsynonymous, splice-site, or insertion/deletion) in 59 genes. Main Outcome Measures Correlation between phenotypes and mutations of interest. Results We detected five rare heterozygous mutations in five different children with obesity: MC4R p.Ile301Thr and SIM1 p.Val326Thrfs*43 mutations that were pathogenic; SIM1 p.Ser343Pro and SH2B1 p.Pro90His mutations that were likely pathogenic; and NTRK2 p.Leu140Phe that was of uncertain significance. In parallel, we identified seven carriers of mutations in ABCC8 (p.Lys1521Asn and p.Ala625Val) or KCNJ11 (p.Val13Met and p.Val151Met) that were of uncertain significance. Conclusions We were able to detect pathogenic or likely pathogenic mutations linked to severe obesity in >15% of this population, which is much higher than what we observed in Europeans (∼5%). We investigated whether rare genetic mutations in 59 genes involved in monogenic obesity or diabetes might be causal in obese children of Afro-Caribbean ancestry (from Guadeloupe Island).

• Publisher: The Endocrine Society,Endocrine Society,Copyright Oxford University Press,Oxford University Press
• Subject: [SDV]Life Sciences [q-bio] / Adolescent / African Continental Ancestry Group - genetics / African Continental Ancestry Group - statistics & numerical data / Black People / Caribbean Region / Caribbean Region - ethnology / Child / Children / Clonal deletion / Cohort Studies / Diabetes / Diabetes mellitus / DNA Mutational Analysis / Female / Gene deletion / Guadeloupe / Guadeloupe - epidemiology / High-Throughput Nucleotide Sequencing / Humans / Insertion / Life Sciences / Male / Mutation / Next-generation sequencing / Obesity / Pediatric Obesity / Pediatric Obesity - epidemiology / Pediatric Obesity - ethnology / Pediatric Obesity - genetics / Phenotypes / Prevalence / Schools / Schools - statistics & numerical data / Students / Students - statistics & numerical data / Studies