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In vitro modeling of respiratory syncytial virus infection of pediatric bronchial epithelium, the primary target of infection in vivo
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In vitro modeling of respiratory syncytial virus infection of pediatric bronchial epithelium, the primary target of infection in vivo
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Journal Article
In vitro modeling of respiratory syncytial virus infection of pediatric bronchial epithelium, the primary target of infection in vivo
2012
Overview
Respiratory syncytial virus (RSV) is the major viral cause of severe pulmonary disease in young infants worldwide. However, the mechanisms by which RSV causes disease in humans remain poorly understood. To help bridge this gap, we developed an ex vivo/in vitro model of RSV infection based on well-differentiated primary pediatric bronchial epithelial cells (WD-PBECs), the primary targets of RSV infection in vivo. Our RSV/WD-PBEC model demonstrated remarkable similarities to hallmarks of RSV infection in infant lungs. These hallmarks included restriction of infection to noncontiguous or small clumps of apical ciliated and occasional nonciliated epithelial cells, apoptosis and sloughing of apical epithelial cells, occasional syncytium formation, goblet cell hyperplasia/metaplasia, and mucus hypersecretion. RSV was shed exclusively from the apical surface at titers consistent with those in airway aspirates from hospitalized infants. Furthermore, secretion of proinflammatory chemokines such as CXCL10, CCL5, IL-6, and CXCL8 reflected those chemokines present in airway aspirates. Interestingly, a recent RSV clinical isolate induced more cytopathogenesis than the prototypic A2 strain. Our findings indicate that this RSV/WD-PBEC model provides an authentic surrogate for RSV infection of airway epithelium in vivo. As such, this model may provide insights into RSV pathogenesis in humans that ultimately lead to successful RSV vaccines or therapeutics.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Babies
/ Bronchi
/ Cells
/ Child
/ Chronic obstructive pulmonary disease
/ Cilia
/ Cytopathogenic Effect, Viral
/ Epithelial Cells - pathology
/ Epithelial Cells - secretion
/ Human respiratory syncytial virus
/ Humans
/ Infants
/ lungs
/ Mucus
/ Respiratory Mucosa - pathology
/ Respiratory Mucosa - virology
/ Respiratory Syncytial Virus Infections
/ Respiratory Syncytial Virus Infections - pathology
/ Respiratory Syncytial Virus Infections - virology
/ Respiratory Syncytial Viruses
/ Respiratory Syncytial Viruses - pathogenicity
/ Respiratory Syncytial Viruses - physiology
/ Vaccines
/ virology
/ Viruses
