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Genomic characterization and pre-clinical evaluation of a new polyvalent lytic Loughborough phage
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Genomic characterization and pre-clinical evaluation of a new polyvalent lytic Loughborough phage
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Journal Article
Genomic characterization and pre-clinical evaluation of a new polyvalent lytic Loughborough phage
2025
Overview
Carbapenem-resistant
Acinetobacter baumannii
(CRAB) has become a critical concern that necessitates the development of novel antimicrobial approaches. One of the most promising innovative approaches for combating CRAB infections is using effective and lytic bacteriophages (phages), known as phage therapy. Therefore, we recovered and characterized a polyvalent lytic Salmonella_phage_VB_ST-SA173, producing lytic activity against 6 CRAB clinical isolates and 3 multidrug-resistant (MDR)
Salmonella
serovars. Throughout pH 2–10, and thermal stability at up to 60 °C, the phage maintained its stability and lytic activity against the tested isolates. The presence of a tailed phage with a characteristic prolate head and a contractile tail was detected by the transmission electron microscope (TEM). According to the Oxford nanopore sequencing data, the genome of Salmonella_phage_VB_ST-SA173 was 53,636 bp in size, contained 45.9% G + C, and had 53 opening reading frames (ORFs). According to the TEM, ORFs, and BLASTn analysis findings, it was proved that the Salmonella_phage_VB_ST-SA173 belongs to the
Loughboroughvirus
genus. The efficacy of the phage-formulated Carbopol 940 hydrogel in wound healing was assessed preclinically in an infected burn wound animal model with a CRABa clinical isolate. The survival rate was enhanced in the phage-treated group compared to the untreated control groups. Histopathological analysis showed improved wound healing in the form of apparently healthy skin with apparently normal epidermal and dermis layers. In conclusion, depending on its in vitro and physicochemical traits, the phage-loaded hydrogel is expected to be a promising candidate for clinical trials against human CRAB-related skin infections.
Key points
•
A polyvalent Loughboroughvirus phage showed lytic activity against CRAB and Salmonella serovars.
•
The phage showed stability at a wide range of pH and temperature.
•
The phage hydrogel enhanced healing in the burn-wound animal model infected with CRABa.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V,Springer
Subject
/ Acinetobacter baumannii - drug effects
/ Acinetobacter baumannii - virology
/ Acinetobacter Infections - microbiology
/ Acinetobacter Infections - therapy
/ Animals
/ Biomedical and Life Sciences
/ CRAB
/ Dermis
/ genome
/ Genomes
/ Genomics
/ genus
/ head
/ Humans
/ Hydrogel
/ Microbial Genetics and Genomics
/ Molecular analysisSalmonella
/ Phages
/ Rats
/ Salmonella Phages - classification
/ Salmonella Phages - genetics
/ Salmonella Phages - isolation & purification
/ Salmonella Phages - physiology
/ Salmonella Phages - ultrastructure
