Asset Details
Do you wish to reserve the book?
Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology
Do you wish to request the book?
Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology
2014
Overview
This protocol describes how to prepare mouse, rat, human and porcine pancreas tissue slices and how to use them to investigate cellular mechanisms underlying the function, pathology and interaction of endocrine and exocrine components of the pancreas.
Studies on the cellular function of the pancreas are typically performed
in vitro
on its isolated functional units, the endocrine islets of Langerhans and the exocrine acini. However, these approaches are hampered by preparation-induced changes of cell physiology and the lack of an intact surrounding. We present here a detailed protocol for the preparation of pancreas tissue slices. This procedure is less damaging to the tissue and faster than alternative approaches, and it enables the
in situ
study of pancreatic endocrine and exocrine cell physiology in a conserved environment. Pancreas tissue slices facilitate the investigation of cellular mechanisms underlying the function, pathology and interaction of the endocrine and exocrine components of the pancreas. We provide examples for several experimental applications of pancreas tissue slices to study various aspects of pancreas cell biology. Furthermore, we describe the preparation of human and porcine pancreas tissue slices for the validation and translation of research findings obtained in the mouse model. Preparation of pancreas tissue slices according to the protocol described here takes less than 45 min from tissue preparation to receipt of the first slices.
Publisher
Nature Publishing Group UK,Nature Publishing Group
