Journal Article

Effect of Lactobacillus acidophilus and Bifidobacterium bifidum supplementation to standard triple therapy on Helicobacter pylori eradication and dynamic changes in intestinal flora

Huang, Ying, Wang, Yu-huan

2014

Overview

To investigate Lactobacillus acidophilus (L. acidophilus) and Bifidobacterium bifidum (B. bifidum) supplementation to triple therapy for Helicobacter pylori (H. pylori) eradication and dynamic changes in intestinal flora in children with H. pylori infection. One hundred H. pylori-infected children were randomly assigned to two groups: treatment group (n = 43), standard triple anti-H. pylori therapy plus probiotics of L. acidophilus and B. bifidum for 2 weeks followed by taking probiotics for another 4 weeks; control group (n = 45), standard triple anti-H. pylori therapy for 6 weeks. After 6-week treatment, ¹³C-urease breath test was performed and side effects were monitored during the observation period. Quantitative PCR with 16S rRNA-gene-targeted species-specific primers was carried out for the analysis of human intestinal B. bifidum, L. acidophilus, and Escherichia coli (E. coli). As expected, treatment group could significantly enhance the H. pylori eradication rate (83.7 vs. 64.4 %, P < 0.05). B. bifidum, L. acidophilus, and E. coli showed no statistical difference before or after therapy in the treatment group. The number of B. bifidum and L. acidophilus was significantly decreased after 2-week treatment in the control group, but after 6-week treatment it significantly increased and nearly returned to the level before treatment. The number of E. coli increased significantly after 2-week treatment, while after 6-week treatment, it nearly decreased to the level before treatment. L. acidophilus and B. bifidum supplementation is effective for H. pylori eradication compared with triple therapy alone.

• Publisher: Springer-Verlag,Springer Netherlands,Springer Nature B.V
• Subject: administration & dosage / adverse effects / Analysis / Anti-Bacterial Agents / Anti-Bacterial Agents - administration & dosage / Antibiotics / Applied Microbiology / Bacteria / Bacterial Load / Bifidobacterium / Bifidobacterium - growth & development / Bifidobacterium - isolation & purification / Bifidobacterium bifidum / Biochemistry / Biological Therapy / Biological Therapy - methods / Biomedical and Life Sciences / Biotechnology / Breath Tests / Child / Child, Preschool / Children / Clinical trials / diagnosis / drug effects / Drug resistance / Drug Therapy, Combination / Drug Therapy, Combination - methods / Dynamics / E coli / Environmental Engineering/Biotechnology / Escherichia coli / Escherichia coli - drug effects / Escherichia coli - growth & development / Escherichia coli - isolation & purification / Female / Flora / Gastroenterology / Gastrointestinal Tract / Gastrointestinal Tract - microbiology / genetics / growth & development / Helicobacter Infections / Helicobacter Infections - diagnosis / Helicobacter Infections - therapy / Helicobacter pylori / Helicobacter pylori - drug effects / Helicobacter pylori - growth & development / Helicobacter pylori - isolation & purification / Humans / Infections / Inflammatory bowel disease / intestinal microorganisms / isolation & purification / Lactobacillus acidophilus / Lactobacillus acidophilus - growth & development / Lactobacillus acidophilus - isolation & purification / Life Sciences / Male / methods / Microbiology / Original Paper / Pharmaceutical sciences / Probiotics / Probiotics - administration & dosage / Purpura / quantitative polymerase chain reaction / Real-Time Polymerase Chain Reaction / RNA, Ribosomal, 16S / RNA, Ribosomal, 16S - genetics / Studies / therapeutics / Therapy / Treatment Outcome / Ulcers / Urease / Urease - analysis