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An optimized acetylcholine sensor for monitoring in vivo cholinergic activity
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Journal Article
An optimized acetylcholine sensor for monitoring in vivo cholinergic activity
2020
Overview
The ability to directly measure acetylcholine (ACh) release is an essential step toward understanding its physiological function. Here we optimized the GRAB
ACh
(GPCR-activation-based ACh) sensor to achieve substantially improved sensitivity in ACh detection, as well as reduced downstream coupling to intracellular pathways. The improved version of the ACh sensor retains the subsecond response kinetics, physiologically relevant affinity and precise molecular specificity for ACh of its predecessor. Using this sensor, we revealed compartmental ACh signals in the olfactory center of transgenic flies in response to external stimuli including odor and body shock. Using fiber photometry recording and two-photon imaging, our ACh sensor also enabled sensitive detection of single-trial ACh dynamics in multiple brain regions in mice performing a variety of behaviors.
A genetically encoded acetylcholine sensor with improved sensitivity allows detection of cholinergic neurotransmission in vivo in the
Drosophila
and mouse brain.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Animals, Genetically Modified
/ Behavior, Animal - physiology
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Biosensing Techniques - methods
/ Brain
/ Cholinergic Agents - pharmacology
/ Green Fluorescent Proteins - genetics
/ Green Fluorescent Proteins - metabolism
/ Humans
/ Mice
/ Mushroom Bodies - metabolism
/ Odor
/ Olfactory Cortex - metabolism
/ Receptor, Muscarinic M3 - genetics
/ Receptor, Muscarinic M3 - metabolism
/ Sensors
