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Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
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Journal Article
Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
2020
Overview
Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over the mesoscale with synaptic resolution. We applied the technology to calcium imaging of entire dendritic spans of neurons as well as neural ensembles within multiple cortical regions over two hemispheres of the awake mouse brain.
Integrating a Bessel focus module into a two-photon fluorescence mesoscope enables high-speed volumetric imaging of neuronal activity in soma, dendrites and spines.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
