Journal Article

Isolation, identification and characterization of Paenibacillus polymyxa CR1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production

Weselowski, Brian, Nathoo, Naeem, Yuan, Ze-Chun, Eastman, Alexander William, MacDonald, Jacqueline

2016

Overview

Background Paenibacillus polymyxa is a plant-growth promoting rhizobacterium that could be exploited as an environmentally friendlier alternative to chemical fertilizers and pesticides. Various strains have been isolated that can benefit agriculture through antimicrobial activity, nitrogen fixation, phosphate solubilization, plant hormone production, or lignocellulose degradation. However, no single strain has yet been identified in which all of these advantageous traits have been confirmed. Results P. polymyxa CR1 was isolated from degrading corn roots from southern Ontario, Canada. It was shown to possess in vitro antagonistic activities against the common plant pathogens Phytophthora sojae P6497 (oomycete), Rhizoctonia solani 1809 (basidiomycete fungus), Cylindrocarpon destructans 2062 (ascomycete fungus), Pseudomonas syringae DC3000 (bacterium), and Xanthomonas campestris 93-1 (bacterium), as well as Bacillus cereus (bacterium), an agent of food-borne illness. P. polymyxa CR1 enhanced growth of maize, potato, cucumber, Arabidopsis , and tomato plants; utilized atmospheric nitrogen and insoluble phosphorus; produced the phytohormone indole-3-acetic acid (IAA); and degraded and utilized the major components of lignocellulose (lignin, cellulose, and hemicellulose). Conclusions P. polymyxa CR1 has multiple beneficial traits that are relevant to sustainable agriculture and the bio-economy. This strain could be developed for field application in order to control pathogens, promote plant growth, and degrade crop residues after harvest.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V
• Subject: Acetic acid / Agriculture / Agrochemicals / Antibiosis / Applied microbiology / Arabidopsis - growth & development / Arabidopsis - microbiology / Ascomycota - pathogenicity / Bacillus cereus - pathogenicity / Bacteria / Biofertilizers / Biofuels / Biofuels - microbiology / Biological Control Agents / Biological Microscopy / Biomass / Biomedical and Life Sciences / Biopesticides / Canada / Cellulose / Corn / Crop residues / Cucumis sativus - growth & development / Cucumis sativus - microbiology / Enzymes / Fertilizers - microbiology / Foodborne diseases / Genes / Genomes / Identification and classification / Indoleacetic Acids - metabolism / Indoleacetic Acids - pharmacology / Life Sciences / Lignin - metabolism / Lignocellulose / Microbiology / Microorganisms / Mycology / Nitrogen / Nitrogen Fixation / Outdoor air quality / Paenibacillus polymyxa - genetics / Paenibacillus polymyxa - isolation & purification / Paenibacillus polymyxa - metabolism / Paenibacillus polymyxa - physiology / Parasitology / Pathogens / Peptides / Pest Control, Biological / Pesticides / Phosphates / Phosphorus - metabolism / Phylogenetics / Phylogeny / Phytophthora - pathogenicity / Plant diseases / Plant Diseases - microbiology / Plant Diseases - prevention & control / Plant growth / Plant Growth Regulators - pharmacology / Plant Roots - microbiology / Pseudomonas syringae - pathogenicity / Research Article / Rhizoctonia - pathogenicity / RNA, Ribosomal, 16S - genetics / Soil Microbiology / Solanum lycopersicum - growth & development / Solanum lycopersicum - microbiology / Solanum tuberosum - growth & development / Solanum tuberosum - microbiology / Sustainable agriculture / Tomatoes / Trees / Virology / Xanthomonas campestris - pathogenicity / Zea mays - growth & development / Zea mays - microbiology