Journal Article

Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes

Papavasiliou, Georgia, Brankov, Jovan G., Li, Chengyue, He, Yusheng, Torres, Veronica C., Sattar, Husain A., Tichauer, Kenneth M., Sinha, Lagnojita

2019

Overview

Lymph node biopsy is a primary means of staging breast cancer, yet standard pathological techniques are time-consuming and typically sample less than 1% of the total node volume. A low-cost fluorescence optical projection tomography (OPT) protocol is demonstrated for rapid imaging of whole lymph nodes in three dimensions. The relatively low scattering properties of lymph node tissue can be leveraged to significantly improve spatial resolution of lymph node OPT by employing angular restriction of photon detection. It is demonstrated through porcine lymph node metastases models that simple filtered-backprojection reconstruction is sufficient to detect and localize 200-μm-diameter metastases (the smallest clinically significant) in 1-cm-diameter lymph nodes.

• Publisher: Society of Photo-Optical Instrumentation Engineers,S P I E - International Society for
• Subject: Animal models / Animals / Aperture / Biopsy / Breast Neoplasms - diagnostic imaging / Breast Neoplasms - pathology / Cancer / Cell Culture Techniques / Cell Line, Tumor / Female / Fluorescence / Green Fluorescent Proteins - metabolism / Humans / Image reconstruction / JBO Letters / Letter / Lymph nodes / Lymph Nodes - pathology / Lymphatic Metastasis - diagnostic imaging / Lymphatic system / Metastases / Metastasis / Microscopy / Monte Carlo simulation / Optical properties / Pathology / Scattering, Radiation / Spatial discrimination / Spatial resolution / Spheroids, Cellular / Swine / Tissues / Tomography / Tomography, Optical - methods