Journal Article

Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma

Whitley, Melodi Javid, Xu, Eric S., Kuo, Hsuan-Cheng, Huang, Jianguo, Lopez, Omar M., Gersbach, Charles A., Chen, Mark, Kirsch, David G., Nelson, Christopher E., Dodd, Rebecca D., Walens, Andrea, Cardona, Diana M., Eward, William C., Reddy, Anupama, Dave, Sandeep S., Luo, Lixia, Mowery, Yvonne M., Van Mater, David, Robinson-Hamm, Jacqueline N., Ma, Yan

2017

Overview

Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been adapted to generate autochthonous tumours in mice, but how these tumours compare to tumours generated by conventional recombinase technology remains to be fully explored. Here we use CRISPR-Cas9 to generate multiple subtypes of primary sarcomas efficiently in wild type and genetically engineered mice. These data demonstrate that CRISPR-Cas9 can be used to generate multiple subtypes of soft tissue sarcomas in mice. Primary sarcomas generated with CRISPR-Cas9 and Cre recombinase technology had similar histology, growth kinetics, copy number variation and mutational load as assessed by whole exome sequencing. These results show that sarcomas generated with CRISPR-Cas9 technology are similar to sarcomas generated with conventional modelling techniques and suggest that CRISPR-Cas9 can be used to more rapidly generate genotypically and phenotypically similar cancers. Site-specific recombination and CRISPR-Cas9 have been used to generate genetically engineered mouse models of cancer. Here the authors compare sarcomas generated using both systems and see similar genetic and cellular phenotypes, suggesting CRISPR-Cas9 can be used to rapidly generate sarcoma models.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/51 / 38/23 / 42/44 / 631/208/4041/3196 / 631/208/514/2254 / 631/67/1798 / 631/67/70 / 64/110 / 64/60 / Animal models / Animals / Copy number / Cre recombinase / CRISPR / CRISPR-Cas Systems / Electroporation / Gene Editing - methods / Genetic engineering / Growth kinetics / Histology / Humanities and Social Sciences / Integrases / Male / Mice / Mice, Nude / multidisciplinary / Mutation / Neurilemmoma - genetics / Neurilemmoma - pathology / NIH 3T3 Cells / Rodents / Sarcoma / Sarcoma, Experimental - genetics / Sarcoma, Experimental - pathology / Science / Science (multidisciplinary) / Sequences / Tumors