Journal Article

Genome and transcriptome analyses of the mountain pine beetle-fungal symbiont Grosmannia clavigera, a lodgepole pine pathogen

Chan, Simon K, Tanguay, Philippe, Holt, Robert A, Hamelin, Richard C, Centre National de la Recherche Scientifique (CNRS), Robertson, Gordon, Taylor, Greg, Marra, Marco A, Haridas, Sajeet, Henrissat, Bernard, Feau, Nicolas, Tsui, Clement K. M, Bohlmann, Jörg, Unité mixte de recherche de biotechnologie des champignons filamenteux ; Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Université de Provence - Aix-Marseille 1, Hirst, Martin, British Columbia Cancer Agency, Docking, Roderick T, Liao, Nancy Y, Breuil, Colette, Levasseur, Anthony, Natural Resources Canada (NRCan), Natural Sciences and Engineering Research Council of Canada; British Columbia Ministry of Forests; Canadian Forest Service Genomics; Genome Canada; Genome British Columbia; Genome Alberta; University of British Columbia; National Center for Biotechnology Information Genome, Birol, Inanç, University of British Columbia [Canada] (UBC), Department of Wood Science ; Universität Hamburg = University of Hamburg (UHH), Biod

2011

Overview

In western North America, the current outbreak of the mountain pine beetle (MPB) and its microbial associates has destroyed wide areas of lodgepole pine forest, including more than 16 million hectares in British Columbia. Grosmannia clavigera (Gc), a critical component of the outbreak, is a symbiont of the MPB and a pathogen of pine trees. To better understand the interactions between Gc, MPB, and lodgepole pine hosts, we sequenced the ∼30-Mb Gc genome and assembled it into 18 supercontigs. We predict 8,314 protein-coding genes, and support the gene models with proteome, expressed sequence tag, and RNA-seq data. We establish that Gc is heterothallic, and report evidence for repeat-induced point mutation. We report insights, from genome and transcriptome analyses, into how Gc tolerates conifer-defense chemicals, including oleoresin terpenoids, as they colonize a host tree. RNA-seq data indicate that terpenoids induce a substantial antimicrobial stress in Gc, and suggest that the fungus may detoxify these chemicals by using them as a carbon source. Terpenoid treatment strongly activated a ∼100-kb region of the Gc genome that contains a set of genes that may be important for detoxification of these host-defense chemicals. This work is a major step toward understanding the biological interactions between the tripartite MPB/fungus/forest system.

• Publisher: National Academy of Sciences,CCSD,National Acad Sciences
• Subject: Animals / Antimicrobial agents / Bark beetles / Biological Sciences / British Columbia / carbon / Carbon sources / Cellular Biology / Chemicals / Coleoptera - microbiology / Coniferous forests / Conifers / Data processing / Dendroctonus ponderosae / Detoxification / Entomology / Enzymes / Evergreen trees / expressed sequence tags / Forests / Fungal Proteins - genetics / Fungi / Gene expression / Genes / Genome, Fungal - genetics / Genome-Wide Association Study / Genomes / Genomics / Grosmannia / hosts / Insects / Life Sciences / Mountains / oleoresins / Ophiostomatales - genetics / Outbreaks / Pathogens / Pest outbreaks / Phloem / Pine trees / Pinus - microbiology / Pinus contorta var. latifolia / Plant Diseases - genetics / Plant Diseases - microbiology / Plant pathology / Point mutation / proteome / Ribonucleic acid / RNA / Sequencing / Stress / Symbionts / Symbiosis - physiology / Terpenoids / Transcription, Genetic - genetics / transcriptome / Trees
• ISBN: 0002870845000