Journal Article

Adhesion of Staphylococcus aureus to Corneocytes from Atopic Dermatitis Patients Is Controlled by Natural Moisturizing Factor Levels

McAleer, Maeve A., Kezic, Sanja, Geoghegan, Joan A., Feuillie, Cécile, Dufrêne, Yves F., Irvine, Alan D., Vitry, Pauline

2018

Overview

The bacterial pathogen Staphylococcus aureus plays an important role in atopic dermatitis (AD), a chronic disorder that mostly affects children. Colonization of the skin of AD patients by S. aureus exacerbates the disease, but the molecular determinants of the bacterium-skin adhesive interactions are poorly understood. Specifically, reduced levels of natural moisturizing factor (NMF) in the stratum corneum have been shown to be associated with more severe AD symptoms, but whether this is directly related to S. aureus adhesion is still an open question. Here, we demonstrate a novel relationship between NMF expression in AD skin and strength of bacterial adhesion. Low-NMF corneocytes, unlike high-NMF ones, are covered by a dense layer of nanoscale villus protrusions. S. aureus bacteria isolated from AD skin bind much more strongly to corneocytes when the NMF level is reduced. Strong binding forces originate from a specific interaction between the bacterial adhesion clumping factor B (ClfB) and skin ligands. Remarkably, mechanical tension dramatically strengthens ClfB-mediated adhesion, as observed with catch bonds, demonstrating that physical stress plays a role in promoting colonization of AD skin by S. aureus . Collectively, our findings demonstrate that patient NMF levels regulate the strength of S. aureus -corneocyte adhesion, the first step in skin colonization, and suggest that the ClfB binding mechanism could represent a potential target for new therapeutic treatments. IMPORTANCE Bacterium-skin interactions play important roles in skin disorders, yet their molecular details are poorly understood. In this study, we decipher the molecular forces at play during adhesion of Staphylococcus aureus to skin corneocytes in the clinically important context of atopic dermatitis (AD), also known as eczema. We identify a unique relationship between the level of natural moisturizing factor (NMF) in the skin and the strength of bacterium-corneocyte adhesion. Bacterial adhesion is primarily mediated by the surface protein clumping factor B (ClfB) and is enhanced by physical stress, highlighting the role of protein mechanobiology in skin colonization. Similar to a catch bond behavior, this mechanism represents a promising target for the development of novel antistaphylococcal agents. Bacterium-skin interactions play important roles in skin disorders, yet their molecular details are poorly understood. In this study, we decipher the molecular forces at play during adhesion of Staphylococcus aureus to skin corneocytes in the clinically important context of atopic dermatitis (AD), also known as eczema. We identify a unique relationship between the level of natural moisturizing factor (NMF) in the skin and the strength of bacterium-corneocyte adhesion. Bacterial adhesion is primarily mediated by the surface protein clumping factor B (ClfB) and is enhanced by physical stress, highlighting the role of protein mechanobiology in skin colonization. Similar to a catch bond behavior, this mechanism represents a promising target for the development of novel antistaphylococcal agents.

• Publisher: American Society for Microbiology
• Subject: Adhesins, Bacterial - metabolism / adhesion / Atopic dermatitis / Bacteria / Bacterial Adhesion / Bacteriology / biofilms / Biomechanics / Colonization / Dermatitis / Dermatitis, Atopic - microbiology / Dermatitis, Atopic - pathology / Dermatology / Disease / Eczema / Human health and pathology / Humans / Infectious diseases / Intermediate Filament Proteins - metabolism / Keratinocytes - microbiology / Life Sciences / Ligands / Mechanics / Microbiology and Parasitology / Microscopy / Morphology / Mutation / Patients / Physics / Protein Binding / Proteins / Skin / Skin - microbiology / Skin diseases / Staphylococcal Infections - microbiology / Staphylococcus aureus / Staphylococcus aureus - physiology / Statistical analysis / Stratum corneum / Villus