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Detection of acute Q fever human cases by indirect immunofluorescence & real-time polymerase chain reaction in a tertiary care hospital in Puducherry
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Detection of acute Q fever human cases by indirect immunofluorescence & real-time polymerase chain reaction in a tertiary care hospital in Puducherry
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Journal Article
Detection of acute Q fever human cases by indirect immunofluorescence & real-time polymerase chain reaction in a tertiary care hospital in Puducherry
2018
Overview
Five IFA positive (including two qPCR positive) and 13 IFA negative patients had a history of domestic animal contact such as cattle, sheep, goat, dog or their residence situated near the abattoir [Table 1]. Laboratory definition and interpretation of acute QF[9] based on gold standard IFA is as follows: (i) IgM phase II + IgG phase II antibodies → acute QF; (ii) IgG phase II with or without IgG phase I → resolved past QF; and (iii) other combination viz., IgM/IgG phase II and/or IgM/IgG phase I → inconclusive, PCR to be performed. [...]the present study showed presence of QF in and around Puducherry. Authors thank the Indian Council of Medical Research (ICMR), New Delhi, for ad-hoc task force research project to the third author (SS) (30/3/41/2008/ECD-II), and to the Chairman, Vice-Chancellor, and Dean (Research and Allied Health Sciences) of Mahatma Gandhi Medical College and Research Institute, Puducherry, for providing financial assistance from Sri Balaji Vidyapeeth University Faculty Research Fund.
Publisher
Wolters Kluwer India Pvt. Ltd,Medknow Publications and Media Pvt. Ltd,Scientific Scholar,Medknow Publications & Media Pvt Ltd
Subject
/ Adult
/ Child
/ Coxiella burnetii - genetics
/ Coxiella burnetii - immunology
/ Coxiella burnetii - isolation & purification
/ DNA
/ Female
/ Fluorescent antibody technique
/ Fluorescent Antibody Technique, Indirect
/ Gandhi, Mohandas Karamchand (1869-1948)
/ Humans
/ Infant
/ Male
/ Pain
/ Patients
/ Q fever
/ R&D
