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Assessment of vine shoots and surplus grape must for succinic acid bioproduction
by
Hijosa-Valsero, María
, Paniagua-García, Ana I.
, Díez-Antolínez, Rebeca
in
Acid production
/ Acids
/ Actinobacillus succinogenes
/ Ammonium chloride
/ Analysis
/ Biomedical and Life Sciences
/ Biotechnological Products and Process Engineering
/ Biotechnology
/ Choline
/ choline chloride
/ enzymatic hydrolysis
/ feedstocks
/ Fermentation
/ Fruits
/ grape must
/ Grapes
/ Hydrolysates
/ Hydrolysis
/ Identification and classification
/ Life Sciences
/ Methods
/ Microbial Genetics and Genomics
/ Microbiology
/ Musts
/ Nitrogen
/ Nitrogen sources
/ Organic nitrogen
/ red wines
/ Shoots
/ Substrate inhibition
/ Succinic acid
/ Sugar
/ sugars
/ Taste
/ Urea
/ vines
/ Vitamins
/ white wines
/ Wine
/ Wines
/ Yeast
/ yeast extract
2022
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Assessment of vine shoots and surplus grape must for succinic acid bioproduction
by
Hijosa-Valsero, María
, Paniagua-García, Ana I.
, Díez-Antolínez, Rebeca
in
Acid production
/ Acids
/ Actinobacillus succinogenes
/ Ammonium chloride
/ Analysis
/ Biomedical and Life Sciences
/ Biotechnological Products and Process Engineering
/ Biotechnology
/ Choline
/ choline chloride
/ enzymatic hydrolysis
/ feedstocks
/ Fermentation
/ Fruits
/ grape must
/ Grapes
/ Hydrolysates
/ Hydrolysis
/ Identification and classification
/ Life Sciences
/ Methods
/ Microbial Genetics and Genomics
/ Microbiology
/ Musts
/ Nitrogen
/ Nitrogen sources
/ Organic nitrogen
/ red wines
/ Shoots
/ Substrate inhibition
/ Succinic acid
/ Sugar
/ sugars
/ Taste
/ Urea
/ vines
/ Vitamins
/ white wines
/ Wine
/ Wines
/ Yeast
/ yeast extract
2022
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Assessment of vine shoots and surplus grape must for succinic acid bioproduction
by
Hijosa-Valsero, María
, Paniagua-García, Ana I.
, Díez-Antolínez, Rebeca
in
Acid production
/ Acids
/ Actinobacillus succinogenes
/ Ammonium chloride
/ Analysis
/ Biomedical and Life Sciences
/ Biotechnological Products and Process Engineering
/ Biotechnology
/ Choline
/ choline chloride
/ enzymatic hydrolysis
/ feedstocks
/ Fermentation
/ Fruits
/ grape must
/ Grapes
/ Hydrolysates
/ Hydrolysis
/ Identification and classification
/ Life Sciences
/ Methods
/ Microbial Genetics and Genomics
/ Microbiology
/ Musts
/ Nitrogen
/ Nitrogen sources
/ Organic nitrogen
/ red wines
/ Shoots
/ Substrate inhibition
/ Succinic acid
/ Sugar
/ sugars
/ Taste
/ Urea
/ vines
/ Vitamins
/ white wines
/ Wine
/ Wines
/ Yeast
/ yeast extract
2022
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Assessment of vine shoots and surplus grape must for succinic acid bioproduction
Journal Article
Assessment of vine shoots and surplus grape must for succinic acid bioproduction
2022
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Overview
Vine shoots and surplus grape must were assessed as feedstocks for succinic acid production with
Actinobacillus succinogenes
and
Basfia succiniproducens
. After acidic and enzymatic hydrolysis, vine shoots released 35–40 g/L total sugars. Both bacterial species produced 18–21 g/L succinic acid from this hydrolysate in 120 h. Regarding grape must fermentation,
A. succinogenes
clearly outperformed
B. succiniproducens
. Yeast extract (a source of organic nitrogen and vitamins) was the only additional nutrient needed by
A. succinogenes
to grow on grape must. Under mathematically optimized conditions (145.7 g/L initial sugars and 24.9 g/L yeast extract),
A. succinogenes
generated 88.9 ± 1.4 g/L succinic acid in 96 h, reaching a succinic acid yield of 0.66 ± 0.01 g/g and a sugar consumption of 96.64 ± 0.30%. Substrate inhibition was not observed in grape musts with 125–150 g/L initial sugars, provided that an adequate amount of yeast extract was available for bacteria. Alternative nitrogen sources to yeast extract (red wine lees, white wine lees, urea, NH
4
Cl, and choline chloride) were not suitable for
A. succinogenes
in grape must.
Key Points
• Vine shoots and surplus grape must were assessed for succinic acid bioproduction.
• Succinic acid bioproduction was 21 g/L with vine shoots and 89 g/L with grape must.
• Fermentation was efficient at high sugar loads if organic N supply was adequate.
Graphical abstract
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Acids
/ Analysis
/ Biomedical and Life Sciences
/ Biotechnological Products and Process Engineering
/ Choline
/ Fruits
/ Grapes
/ Identification and classification
/ Methods
/ Microbial Genetics and Genomics
/ Musts
/ Nitrogen
/ Shoots
/ Sugar
/ sugars
/ Taste
/ Urea
/ vines
/ Vitamins
/ Wine
/ Wines
/ Yeast
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