Journal Article

Long-term in vivo vitamin D3 supplementation modulates bovine IL-1 and chemokine responses

Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias [Mexico] (INIFAP), Remot, Aude, Kennedy, Emer, Infectiologie et Santé Publique (ISP) ; Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), School of Medicine, Trinity College ; Trinity College Dublin, Conway Institute of Biomolecular and Biomedical Research [Dublin, Irlande] ; University College Dublin [Dublin] (UCD), Trinity College Dublin, European Project: 731014,VetBioNet, Animal & Grassland Research and Innovation Centre ; Teagasc - The Agriculture and Food Development Authority (Teagasc), Animal & Grassland Research and Innovation Centre,Teagasc, Moorepark ; Animal and Grassland Research and Innovation Centre (AGRICE), Meade, Kieran, G, Institute of Food and Health, Reid, Cian, O’farrelly, Cliona, Funding for the animal trial study was provided via a Science Foundation Ireland grant to Kieran Meade (17/CDA/4717). This work was also funded by a Teagasc Walsh Scholarship a

2023

Overview

Vitamin D deficiency at birth, followed by prolonged insufficiency in early life may predispose bovine calves to infection and disease. However, the effects of vitamin D levels on innate immunity are unclear due to the lack of long-term supplementation trials in vivo and reliable approaches for reproducibly assessing immune function. Here, a standardized whole blood immunophenotyping assay was used to compare innate immune responses to infection relevant ligands (LPS, Pam3CSK4 and R848) between Holstein–Friesian calves supplemented with vitamin D (n = 12) from birth until 7 months of age and control calves (n = 10) raised on an industry standard diet. Transcriptomic analysis in unstimulated whole blood cells revealed increased expression of type I interferons and chemokines in vitamin D supplemented calves, while IL-1 and inflammasome gene expression was decreased. In response to stimulation with the bacterial ligand LPS, supplemented calves had significantly increased expression of CASP1 , CX3CR1 , CAT , whereas STAT1 was decreased . Stimulation with the bacterial ligand Pam3CSK4 revealed increased expression of IL1A , IL1B and CAT genes; and decreased C5AR1 expression. In response to the viral ligand R848, STAT1 and S100A8 expression was significantly decreased. An increased IL-1 and inflammasome gene expression signature in vitamin D supplemented calves in response to LPS and Pam3CSK4 was also found, with ELISA confirming increased IL-1β protein production. In contrast, a decreased chemokine gene expression signature was found in response to R848 in supplemented animals, with decreased IL-8 protein expression exhibited in response to all PAMPs also found. These results demonstrated expression of several cytokine, chemokine and inflammasome genes were impacted by vitamin D supplementation in the first 7 months of life, with IL-8 expression particularly responsive to vitamin D. Overall, vitamin D supplementation induced differential innate immune responses of blood immune cells that could have important implications for disease susceptibility in cattle.

• Publisher: Nature Publishing Group,CCSD,Nature Publishing Group UK,Nature Portfolio
• Subject: 631/250/262 / 631/250/98 / Animal biology / Blood cells / Chemokines / CX3CR1 protein / Enzyme-linked immunosorbent assay / Feed additives / Gene expression / Humanities and Social Sciences / Immune response / Immunology / Inflammasomes / Innate immunity / Interleukin 1 / Interleukin 8 / Life Sciences / Ligands / Lipopolysaccharides / multidisciplinary / Science / Science (multidisciplinary) / Stat1 protein / Transcriptomics / Vitamin D / Vitamin D3 / Vitamin deficiency
• ISBN: 0010246004000