Asset Details
Do you wish to reserve the book?
Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell
Do you wish to request the book?
Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell
2000
Overview
We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[
a
]pyrene (BaP) and of the BaP–DNA adduct. Detection of BPT is of great biomedical interest, since this species can serve as a biomarker for monitoring DNA damage due to BaP exposure and for possible precancer diagnosis. The measurements were performed on the rat liver epithelial clone 9 cell line, which was used as the model cell system. Before making measurements, the cells were treated with BPT. Nanoprobes were inserted into individual cells, incubated 5 min to allow antigen–antibody binding, and then removed for fluorescence detection. We determined a concentration of 9.6 ± 0.2 × 10
−11
M for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe.
Publisher
Nature Publishing Group US,Nature,Nature Publishing Group
Subject
/ Animals
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Cloning
/ DNA
/ Fluorescent Antibody Technique - instrumentation
/ Fundamental and applied biological sciences. Psychology
/ Methods. Procedures. Technologies
/ Microscopy, Electron, Scanning
/ Pyrene
/ Rats
/ Silver
/ Spectrometry, Fluorescence - instrumentation
