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Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology
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Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology
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Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology
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Journal Article
Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology
2011
Overview
Modular and orthogonal genetic logic gates are essential for building robust biologically based digital devices to customize cell signalling in synthetic biology. Here we constructed an orthogonal AND gate in
Escherichia coli
using a novel hetero-regulation module from
Pseudomonas syringae
. The device comprises two co-activating genes
hrpR
and
hrpS
controlled by separate promoter inputs, and a σ
54
-dependent
hrpL
promoter driving the output. The
hrpL
promoter is activated only when both genes are expressed, generating digital-like AND integration behaviour. The AND gate is demonstrated to be modular by applying new regulated promoters to the inputs, and connecting the output to a NOT gate module to produce a combinatorial NAND gate. The circuits were assembled using a parts-based engineering approach of quantitative characterization, modelling, followed by construction and testing. The results show that new genetic logic devices can be engineered predictably from novel native orthogonal biological control elements using quantitatively in-context characterized parts.
Biological digital sensors require the fabrication of modular genetic logic gates. Using the
Pseudomonas syringae hrp
system, Wang and colleagues generate AND, NOT and NAND gates, demonstrating the ability to engineer a modular system from biological elements.
