Journal Article

Changes of CD8+CD28- Tregs and Gamma-Delta-T-cells in a Neonate with Intrauterine Cytomegalovirus Infection: A Case Report

Zhou, Ping, Tang, Quan, Chen, Xian, Jin, Zhenchao, Jiang, Fan, Xie, Tingyan

2024

Overview

Background Congenital cytomegalovirus (cCMV) infection can lead to a range of adverse outcomes. The majority of cCMV neonates with clinical symptoms are infected postnatally; however, established cases of intrauterine infection are uncommon, resulting in a paucity of reports on clinical findings and lymphocytes expression in CMV-infected neonates. Case presentation We followed a neonate with cCMV infection from the onset of hospitalization to several months of follow-up. This infant was intrauterine CMV-positive in the amniotic fluid of the mother at 21 weeks’ gestation and received intravenous ganciclovir infusion and sequential oral valganciclovir after birth. The typical clinical signs manifested in the nervous system, liver, and peripheral blood and were documented during the hospitalizaion period and up to the follow-up visit. Flow cytometry was employed to examine the expression of T cells, their subsets, and the associated cytokines in peripheral blood samples at various time points. The flow data for the cCMV neonate were compared with those of the controls at each time point. Following treatment, clinical symptoms improved and the infant became CMV negative. However, developmental delays occurred later in life. The proportion of CD8 + CD28 − Tregs in the peripheral blood of the neonate with congenital CMV infection was higher than that in the controls at the three time points. The expression levels of perforin and granzyme B secreted by γδ T cells (Vδ1 and Vδ2 T cells), increased during the course of hospitalization until follow-up and were higher than those in the controls at the three time points. Conclusions Despite the alleviation of clinical symptoms, developmental delay in later life remains inevitable in this intrauterine cCMV neonate. CD8 + CD28 − Tregs and Vδ1 and Vδ2 T cells secreting perforin and granzyme B may be involved in congenital CMV infection, although this hypothesis requires validation in a larger study. This report may contribute to our understanding of the effect of current treatment and the immune status of intrauterine cCMV-infected neonates.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Acids / Amniotic fluid / Antiviral Agents - therapeutic use / B cells / Blood platelets / Case Report / Case reports / CD28 Antigens / CD8 lymphocytes / CD8+CD28− Tregs / CD8-Positive T-Lymphocytes - immunology / Cerebrospinal fluid / Complications and side effects / Congenital CMV infection / Cytomegalovirus / Cytomegalovirus infections / Cytomegalovirus Infections - diagnosis / Cytomegalovirus Infections - drug therapy / Cytomegalovirus Infections - immunology / Development and progression / Diseases / Female / Fetuses / Flow cytometry / Ganciclovir / Ganciclovir - therapeutic use / Gestational age / Granzymes - metabolism / Hands / Health aspects / Hematology / Humans / Infant, Newborn / Infants (Newborn) / Infection / Infections / Internal Medicine / Liver / Lungs / Lymphocytes / Male / Medical research / Medicine / Medicine & Public Health / Medicine, Experimental / Neonate / Pediatric research / Pediatrics / Perforin - metabolism / Perinatal infection / Physiological aspects / Pregnancy / Pregnancy Complications, Infectious / Suppressor cells / T cells / T-Lymphocytes, Regulatory - immunology / Tumor necrosis factor-TNF / Urine / Vδ1 and Vδ2 T cells / Γδ T cells